Role of cytochrome P sub 450 in the control of the production of erythropoietin

Journal Article · Sun Dec 31 23:00:00 EST 1989 · Life Sciences; (USA)

DOI:https://doi.org/10.1016/0024-3205(90)90225-G· OSTI ID:6533251

Fandrey, J; Seydel, F P; Siegers, C P; Jelkmann, W ^[1]

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1. Medical Univ. of Luebeck (West Germany)

Effects of agents affecting cytochrome P{sub 450} were studied on the production of erythropoietin (Epo) in cultures of the human hepatoma cell line HepG2. Epo was measured by radioimmunoassay of the culture media after 24 h of incubation. The addition of phenobarbital or 3-methylcholanthrene, which induce cytochrome P{sub 450}, significantly enhanced the formation of Epo. Likewise, the thyroid hormones T{sub 3} and T{sub 4} stimulated the rate of the production of Epo. On the other hand, the formation of Epo was lowered following the addition of diethyl-dithiocarbamate or cysteamine chloride, which inhibit cytochrome P{sub 450}. These findings support the idea that O{sub 2} sensitive hemoproteins of the microsomal mixed-functional oxidases play a role in the control of the synthesis of Epo.

• OSTI ID: 6533251
• Journal Information: Life Sciences; (USA), Journal Name: Life Sciences; (USA) Vol. 47:2; ISSN 0024-3205; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

3-METHYLCHOLANTHRENE
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANESTHETICS
ANIMAL CELLS
ANIMALS
ANTICONVULSANTS
AROMATICS
AZINES
BARBITURATES
BIOASSAY
BIOLOGICAL EFFECTS
BIOLOGICAL FUNCTIONS
BIOSYNTHESIS
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
DIAGNOSTIC TECHNIQUES
DISEASES
DRUGS
ENZYME INDUCTION
ENZYME INHIBITORS
ENZYMES
ERYTHROPOIETIN
FUNCTIONS
GENE REGULATION
HEPATOMAS
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROCARBONS
HYPNOTICS AND SEDATIVES
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
MAMMALS
MAN
MICROSOMES
MITOGENS
MIXED-FUNCTION OXIDASES
NEOPLASMS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANOIDS
OXIDOREDUCTASES
OXYGENASES
PEPTIDE HORMONES
PHENOBARBITAL
POLYCYCLIC AROMATIC HYDROCARBONS
PRIMATES
PROTEINS
PYRIMIDINES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
RIBOSOMES
SYNTHESIS
THYROID HORMONES
TRACER TECHNIQUES
TUMOR CELLS
VERTEBRATES