Direct observation of the phase behavior of the lipid bilayers of phage PM2 and the intact host cells by sup 1 H- sup 31 P cross-polarization NMR
- Osaka Univ. (Japan)
A method for obtaining the {sup 31}P NMR spectrum of a particular supramolecular structure in an intact biological system was developed by applying the 1H-31P cross-polarization technique to a lipid-containing bacteriophage, PM2, and its host bacterium, Alteromonas espejiana. It was shown that {sup 31}P NMR spectra of nucleic acids and lipid bilayers can be obtained separately with short and long thermal contact times, respectively. The temperature dependence of the chemical shift anisotropy (delta sigma = sigma parallel - sigma perpendicular) was examined for the separately obtained membrane spectra. Referring to the results of thermal analysis and {sup 31}P NMR spectra of bilayers of the extracted phospholipids, the phase transition of the biomembrane was identified for the PM2 phage and the host cell. The dynamic state of the biomembrane of the intact bacterium was directly monitored in detail. The phase behavior of the PM2 lipid bilayer showed good agreement with an earlier report. It turned out that the phase behavior of the intact biomembrane is different from that of the bilayer of the extracted lipids for both PM2 and the host cell. Namely, the terminal temperatures of the phase transition of the host cell and PM2 membranes were lower and higher than those of the extracted phospholipids, respectively.
- OSTI ID:
- 6532260
- Journal Information:
- Biochemistry; (USA), Journal Name: Biochemistry; (USA) Vol. 29:25; ISSN 0006-2960; ISSN BICHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BACTERIOPHAGES
BARYONS
CELL CONSTITUENTS
CELL MEMBRANES
ELEMENTARY PARTICLES
ESTERS
FERMIONS
HADRONS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPIDS
LIPOSOMES
MAGNETIC RESONANCE
MEMBRANES
MICROORGANISMS
MOLECULAR STRUCTURE
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
NUCLEI
NUCLEONS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANOIDS
PARASITES
PHOSPHOLIPIDS
PHOSPHORUS 31
PHOSPHORUS ISOTOPES
PROTONS
RESONANCE
RIBOSOMES
SPECTRA
STABLE ISOTOPES
TEMPERATURE DEPENDENCE
TRACER TECHNIQUES
VIRUSES