Uncoupled regulation of fibronectin and collagen synthesis in Rous sarcoma virus transformed avian tendon cells

Journal Article · Sat Dec 01 04:00:00 EST 1979 · J. Biol. Chem.; (United States)

OSTI ID:6530709

Parry, G; Soo, W J; Bissell, M J

The regulation of fibronectin and procollagen synthesis has been investigated in normal and Rous sarcoma virus transformed primary avian tendon cells. These two proteins interact at the cell periphery and both are reportedly lost upon transformation. Whether their synthesis was coordinately regulated in Rous sarcoma virus-infected cells was thus examined. It was found that while the synthesis of both pro ..cap alpha../sub 1/ and pro ..cap alpha../sub 2/ peptides was reduced upon transformation, the synthesis of fibronectin was not altered. Nevertheless, long term radiolabeling demonstrated that fibronectin levels were reduced in transformed cells. It is concluded that the reduction in levels of these components at the surface is brought about by different mechanisms; collagen levels being regulated by procollagen synthesis and fibronectin levels by degradation and/or release into the culture medium. The possibility is discussed that fibronectin is lost from the cell periphery of primary avian tendon cells as a consequence of decreased levels of anchoring collagen molecules.

• Research Organization: Univ. of California, Berkeley
• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 6530709
• Journal Information: J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 254:23; ISSN JBCHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
550301 -- Cytology-- Tracer Techniques
550701 -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AUTORADIOGRAPHY
BIOCHEMISTRY
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
BIRDS
CARBON 14 COMPOUNDS
CELL CULTURES
CHEMISTRY
COLLAGEN
ELECTROPHORESIS
LABELLED COMPOUNDS
MICROORGANISMS
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PROTEINS
QUANTITY RATIO
SCLEROPROTEINS
SENSITIVITY
SYNTHESIS
VERTEBRATES
VIRUSES