Primary avian tendon cells in culture: an improved system for understanding malignant transformation

Journal Article · Fri Dec 01 04:00:00 EST 1978 · J. Cell Biol.; (United States)

DOI:https://doi.org/10.1083/jcb.79.3.672· OSTI ID:5415084

Schwarz, R I; Farson, D A; Soo, W J; Bissell, M J

Primary avian tendon (PAT) cells which maintain their differentiated state in culture are rapidly transformed by Rous sarcoma virus. By criteria of morphology, increased rate of 2-deoxyglucose uptake, and loss of density dependent growth control, PAT cells transform as well as their less differentiated counterpart, chick embryo fibroblasts. In addition, the percentage of collagen produced by PAT cells drops on transformation by an order of magnitude, from 23 to 2.5%, but is unaffected by viral replication of a transformation-defective mutant. The responsiveness of normal and transformed PAT cells to various environmental factors changes dramatically upon transformation. Normal PAT cells respond to the presence of ascorbate and high cell density by raising the level of collagen synthesis from 5 to 23%. Transformed PAT cells are totally unresponsive. These and previously reported results lead us to postulate that the breakdown in the normal regulatory mechanisms used by the cell to maintain the differentiated state is related to or is responsible for the onset of malignant transformation.

• Research Organization: Univ. of California, Berkeley
• OSTI ID: 5415084
• Journal Information: J. Cell Biol.; (United States), Journal Name: J. Cell Biol.; (United States) Vol. 79; ISSN JCLBA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ADENOVIRUS
ANIMAL CELLS
ANIMALS
ASCORBIC ACID
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BIRDS
CELL CULTURES
CHEMISTRY
CHICKENS
COLLAGEN
CONNECTIVE TISSUE CELLS
DISEASES
FIBROBLASTS
FOWL
MICROORGANISMS
MORPHOLOGY
MUTATIONS
NEOPLASMS
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PROTEINS
SARCOMAS
SCLEROPROTEINS
SOMATIC CELLS
SYNTHESIS
VERTEBRATES
VIRUSES
VITAMINS