Production of toluene cis-glycol by Pseudomonas putida in glucose fed-batch culture

Journal Article · Fri May 01 04:00:00 EDT 1987 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260290709· OSTI ID:6527043

Jenkins, R O; Stephens, G M; Dalton, H

Toluene was oxidized by a mutant strain of Pseudomonas putida (strain NG1) to toluene cis-glycol (TCG). Product was accumulated in fed-batch cultures to concentrations (18-24 g/L) higher than hitherto achieved. In vitro activities of toluene dioxygenase from P. putida NG1 were fivefold lower than that from the toluene-grown wild-type organism, whereas comparable activities of both catechol 2,3- and catechol 1,2-oxygenase were obtained; irreversible inhibition of toluene dioxygenase activity by TCG was shown in vitro. Ammonia deprivation during the production phase limited the growth of revertant organisms but had little effect on either the duration (25 h) of the process or the final concentration of TCG achieved. The rates of glucose utilization decreased throughout the biotransformation and cell death accompanied the cessation of TCG accumulation in cultures. The results suggest that TCG is the mediator of a gradual deterioration in the state of the culture which leads to a loss of both in vivo and in vitro toluene dioxygenase activity and a marked decrease in culture viability.

• Research Organization: Univ. of Warwick, Coventry, England
• OSTI ID: 6527043
• Journal Information: Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 29:7; ISSN BIBIA
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
090222* -- Alcohol Fuels-- Preparation from Wastes or Biomass-- (1976-1989)
ALCOHOLS
ALDEHYDES
ALKYLATED AROMATICS
AROMATICS
BACTERIA
BATCH CULTURE
CARBOHYDRATES
ENZYME ACTIVITY
ENZYMES
GLUCOSE
GLYCOLS
GROWTH
HEXOSES
HYDROCARBONS
HYDROXY COMPOUNDS
MICROORGANISMS
MONOSACCHARIDES
MUTANTS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
OXYGENASES
PSEUDOMONAS
SACCHARIDES
TOLUENE
YIELDS