Localization of a gene involved in complementation of the defect in xeroderma pigmentosum group A cells on human chromosome 1

Journal Article · Wed Apr 01 04:00:00 EST 1987 · Exp. Cell Res.; (United States)

DOI:https://doi.org/10.1016/0014-4827(87)90209-6· OSTI ID:6526436

Keijzer, W; Stefanini, M; Bootsma, D; Verkerk, A; Geurts van Kessel, A H; Jongkind, J F; Westerveld, A

Human, Chinese hamster or Chinese hamster/human hybrid cytoplasts were fused with UV-irradiated xeroderma pigmentosum group A (XP-A) cells. Unscheduled DNA synthesis (UDS) of the XP-A nucleus was measured 0-2 and 2-4 h after seeding of the fused population. Human cytoplasts did correct the defect in the XP-A nucleus immediately after fusion, whereas the chinese hamster cytoplasts did not show this rapid increase in excision repair. The results obtained after fusion of cytoplasts isolated from a panel of 26 Chinese hamster-human hybrids showed that chromosome 1 bears genetic information that is necessary for the rapid correction of the XP-A defect. Furthermore, this genetic information was regionally assigned to 1q42-qter by analysing hybrid cell lines having retained various segments of chromosome 1. Cytoplasts from a Chinese hamster/XP-A hybrid containing chromosome 1 of XP-A origin corrected also the defect with fast kinetics. This result indicates that the correcting factor consists of human and Chinese hamster components. As a consequence, the gene mapped on chromosome 1 may not be the gene which is mutated in XP-A cells.

• Research Organization: Erasmus Univ., Rotterdam, The Netherlands
• OSTI ID: 6526436
• Journal Information: Exp. Cell Res.; (United States), Journal Name: Exp. Cell Res.; (United States) Vol. 2; ISSN ECREA
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOLOGY
BODY
CONNECTIVE TISSUE CELLS
DISEASES
DNA REPAIR
DNA REPLICATION
FIBROBLASTS
GENE MUTATIONS
GENES
GENETIC MAPPING
GENETICS
HYBRIDIZATION
MAPPING
MUTATIONS
NUCLEIC ACID REPLICATION
ORGANS
RECOVERY
REPAIR
SKIN
SKIN DISEASES
SOMATIC CELLS
XERODERMA PIGMENTOSUM
XP CELLS