Repair-deficient xeroderma pigmentosum cells made UV light resistant by fusion with X-ray-inactivated Chinese hamster cells
Journal Article
·
· Mol. Cell. Biol.; (United States)
Xeroderma pigmentosum (XP) is an autosomal recessive human disease, characterized by an extreme sensitivity to sunlight, caused by the inability of cells to repair UV light-induced damage to DNA. Cell fusion was used to transfer fragments of Chinese hamster ovary (CHO) chromosomes into XP cells. The hybrid cells exhibited UV resistance and DNA repair characteristics comparable to those expressed by CHO cells, and their DNA had greater homology with CHO DNA than did the DNA from XP cells. Control experiments consisted of fusion of irradiated and unirradiated XP cells and repeated exposure of unfused XP cells to UV doses used for hybrid selection. These treatments did not result in an increase in UV resistance, repair capability, or homology with CHO DNA. The hybrid cell lines do not, therefore, appear to be XP revertants. The establishment of these stable hybrid cell lines is an initial step toward identifying and cloning CHO DNA repair genes that complement the XP defect in human cells. The method should also be applicable to cloning genes for other diseases, such as ataxia-telangiectasia and Fanconi's anemia.
- Research Organization:
- Univ. of California, San Francisco
- OSTI ID:
- 6841391
- Journal Information:
- Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 10; ISSN MCEBD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CHO CELLS
CHROMOSOMES
DISEASES
DNA REPAIR
DNA REPLICATION
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
GENES
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
HAMSTERS
IONIZING RADIATIONS
MAMMALS
NUCLEIC ACID REPLICATION
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY
RECOVERY
REPAIR
RODENTS
SKIN DISEASES
SURVIVAL TIME
ULTRAVIOLET RADIATION
VERTEBRATES
X RADIATION
XERODERMA PIGMENTOSUM
XP CELLS
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CHO CELLS
CHROMOSOMES
DISEASES
DNA REPAIR
DNA REPLICATION
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
GENES
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
HAMSTERS
IONIZING RADIATIONS
MAMMALS
NUCLEIC ACID REPLICATION
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY
RECOVERY
REPAIR
RODENTS
SKIN DISEASES
SURVIVAL TIME
ULTRAVIOLET RADIATION
VERTEBRATES
X RADIATION
XERODERMA PIGMENTOSUM
XP CELLS