Radioimmunoimaging of human small cell lung carcinoma xenografts in nude mice receiving several monoclonal antibodies
Small cell carcinoma of the lung is a highly lethal disease, killing nearly all patients afflicted with it. Although it metastasizes early and widely, it is exquisitely radiosensitive, and transient responses to this form of therapy are often dramatic. We have recently developed several monoclonal antibodies (MAb) reactive with antigens associated with this tumor. Scintigraphic and dual-label evaluations of their localization in nude mice bearing small cell carcinoma xenografts (derived from the human small cell carcinoma cell line NCI H69) were undertaken as a preliminary step in the eventual evaluation of their therapeutic potential. Anti-small cell MAb UM-MS1, 2, and 3, were labeled with /sup 131/I with the Iodobead method. Labeling efficiencies ranged from 68% to 88%. A control MAb, 225.28S (antimelanoma), was labeled with /sup 125/I by the same method. Although the labeled anti-small cell antibodies bound 20-40 times more to NCI H69 cells than did the antimelanoma control antibody, their total immunoreactive fraction was relatively low. Separate groups of nude mice bearing NCI H69-derived tumors were studied for imaging and localization with each /sup 131/I-labeled MAb. Successful imaging was achieved with two of the MAb; the quality of the images ranged from moderate to excellent. However, much of the localization to the tumors was due to nonspecific factors, as evidenced by the considerable tumor accretion of the control antibody.
- Research Organization:
- Immunex Corp., Seattle, WA
- OSTI ID:
- 6523822
- Journal Information:
- NCI Monogr.; (United States), Journal Name: NCI Monogr.; (United States) Vol. 3; ISSN NCIME
- Country of Publication:
- United States
- Language:
- English
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550604 -- Medicine-- Unsealed Radionuclides in Therapy-- (1980-)
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL LOCALIZATION
BODY
CARCINOMAS
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSIS
DIAGNOSTIC TECHNIQUES
DIAGNOSTIC USES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE 131
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLING
LUNGS
MAMMALS
MEDICINE
MICE
MONOCLONAL ANTIBODIES
NEOPLASMS
NUCLEAR MEDICINE
NUCLEI
ODD-EVEN NUCLEI
ORGANS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOIMMUNOTHERAPY
RADIOISOTOPE SCANNING
RADIOISOTOPES
RADIOLOGY
RADIOTHERAPY
RESPIRATORY SYSTEM
RODENTS
SCINTISCANNING
THERAPEUTIC USES
THERAPY
TRACER TECHNIQUES
TRANSPLANTS
USES
VERTEBRATES