Mitotic chromosome transmission fidelity mutants in Saccharomyces cerevisiae

Journal Article · Thu Feb 01 04:00:00 EST 1990 · Genetics; (USA)

OSTI ID:6523694

Spencer, F; Gerring, S L; Connelly, C; Hieter, P ^[1]

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1. Johns Hopkins Medical School, Baltimore, MD (USA)

The authors have isolated 136 independent EMS-induced mutations in haploid yeast strains that exhibit decreased chromosome transmission fidelity in mitosis. Eight-five percent of the mutations are recessive and 15% are partially dominant. Complementation analysis between MATa and MAT{alpha} isolates identifies 11 chromosome transmission fidelity (CTF) complementation groups, the largest of which is identical to CHL1. For 49 independent mutations, no corresponding allele has been recovered in the opposite mating type. The initial screen monitored the stability of a centromere-linked color marker on a nonessential yeast chromosome fragment; the mitotic inheritance of natural yeast chromosome III is also affected by the ctf mutations. Of the 136 isolates identified, seven were inviable at 37{degree} and five were inviable at 11{degree}. In all cases tested, these temperature conditional lethalities cosegregated with the chromosome instability phenotype. Five additional complementation groups (ctf12 through ctf16) have been defined by complementation analysis of the mutations causing inviability at 37{degree}. All of the mutant strains showed normal sensitivity to ultraviolet and {gamma}-irradiation.

• OSTI ID: 6523694
• Journal Information: Genetics; (USA), Journal Name: Genetics; (USA) Vol. 124:2; ISSN 0016-6731; ISSN GENTA
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
560300 -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
CELL DIVISION
CHROMOSOMAL ABERRATIONS
CHROMOSOME BREAKAGE
CHROMOSOMES
ELECTROMAGNETIC RADIATION
EMS
ESTERS
EUMYCOTA
FUNGI
GAMMA RADIATION
GENETIC EFFECTS
IONIZING RADIATIONS
MICROORGANISMS
MITOSIS
MUTAGENS
MUTANTS
MUTATIONS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PLANTS
RADIATIONS
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SULFONIC ACID ESTERS
ULTRAVIOLET RADIATION
YEASTS