Cytochrome P/sub 450/ dependent monooxygenases in nasal epithelial membranes: effect of phenobarbital and benzo(a)pyrene

Journal Article · Fri Jan 01 04:00:00 EST 1982 · Proc. West. Pharmacol. Soc.; (United States)

OSTI ID:6521495

Hadley, W M; Dahl, A R; Benson, J M; Hahn, F F; McClellan, R O

The cytochrome P-450 content of microsomes isolated from nasal membranes, lungs, and livers of the mouse, rat, guinea pig, hamster, rabbit, and dog was measured. The cytochrome P-450 dependent monooxygenase activity (MO) was assayed in microsomes from dog nasal membrane, lung, and liver using aniline, p-nitroanisole, aminopyrine and hexamethylphosphoramide (HMPA) as substrates. Phenobarbital was administered as a saturated drinking water solution for 8 days or as an aerosol for 30 minutes on 4 successive days. Benzo(a)pyrene was administered as an aerosol for 1 h on 4 successive days. The drinking water was removed 12 h before sacrifice and the last aerosol exposure was 24 h before sacrifice. All species nasal membranes contained cytochrome P-450. The hamster nasal membranes contained the highest concentrations. All substrates used were metabolized (as measured by MO activity) by the nasal membrane microsomes except for aminopyrine by the maxilloturbinate microsomes. HMPA was the most rapidly metabolized substrate. Liver cytochrome P-450 was induced more than 2 times by the phenobarbital administered in the drinking water, but no induction of nasal membrane cytochrome P-450 was found. Neither the phenobarbital nor benzo(a)pyrene aerosols induced the liver, lung, or nasal membrane cytochrome P-450 content. (RJC)

• Research Organization: Lovelace Inhalation Toxicology Research Inst., Albuquerque, NM
• DOE Contract Number: AC04-76EV01013
• OSTI ID: 6521495
• Journal Information: Proc. West. Pharmacol. Soc.; (United States), Journal Name: Proc. West. Pharmacol. Soc.; (United States) Vol. 25; ISSN PWPSA
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANESTHETICS
ANIMAL TISSUES
ANIMALS
ANTICONVULSANTS
AROMATICS
AZINES
BARBITURATES
BEAGLES
BENZOPYRENE
BODY
BODY AREAS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CONDENSED AROMATICS
DATA
DIGESTIVE SYSTEM
DOGS
DRUGS
ENZYMES
EPITHELIUM
EXPERIMENTAL DATA
FACE
GLANDS
GUINEA PIGS
HAMSTERS
HEAD
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
HYPNOTICS AND SEDATIVES
INFORMATION
INHALATION
INTAKE
LIVER
LUNGS
MAMMALS
METABOLISM
MICE
MIXED-FUNCTION OXIDASES
NOSE
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANS
OXIDOREDUCTASES
OXYGENASES
PHENOBARBITAL
PYRIMIDINES
RABBITS
RATS
RESPIRATORY SYSTEM
RODENTS
TISSUES
VERTEBRATES