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Role of cytochrome B in the processing of the subunits of complex III in the yeast mitochondria

Thesis/Dissertation ·
OSTI ID:6521035

The work described in this dissertation deals with the effect of cytochrome b on the biogenesis and assembly of the subunits of complex III in the mitochondrial membrane of the yeast Saccharomyces cerevisiae. The cytochrome b-mutants (Box mutants of S. cerevisiae form an excellent system to study such a role of cytochome B. The amounts of cytochrome c/sub 1/ in the mitochrondria, as determined both spectroscopically and immunologically, were not affected by the absence of cytochrome b. Pulse labelling of the cells with (/sup 35/S) methionine in the presence of CCCP showed the accumulation of the precursors to the core protein I and the iron-sulfur protein in similar amounts in the mutant Box 6-2 and the wild type cells. Synthesis of the iron sulfur protein and the cytochrome c/sub 1/ by in vitro translation of mRNA isolated from wild type and mutant Box 6-2 in a rabbit reticulocyte lysate system, also confirmed that the synthesis of the nuclear encoded subunits was not affected in the mutants. Pulse labeling of the cells in the absence of CCCP and subsequent chase with cold methionine, however, showed much less of the mature subunits of core protein I and the iron-sulfur protein in the mitochrondria of the mutant cells relative to the wild type. These results indicate that cytochrome b is necessary for the proper processing of certain subunits of complex III.

Research Organization:
City Univ. of New York, NY (USA)
OSTI ID:
6521035
Country of Publication:
United States
Language:
English

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Related Subjects

550701* -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOSYNTHESIS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMISTRY
CYTOCHROMES
DAYS LIVING RADIOISOTOPES
DRUGS
EVEN-ODD NUCLEI
FUNGI
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPOTROPIC FACTORS
MEMBRANES
MESSENGER-RNA
METHIONINE
MICROORGANISMS
MITOCHONDRIA
MUTANTS
NUCLEI
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANOIDS
PIGMENTS
PLANTS
PROTEINS
RADIOISOTOPES
RNA
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
YEASTS