Preferential translation of chloroplast ribosomal proteins in Chlamydomonas reinhardtti
The nuclear cr-1 mutant of C. reinhardtii is deficient in the 30S subunit of the chloroplast (cp) ribosome and in cp protein synthesis. The cp spectinomycin resistant mutant, spr-u-1-27-3, has a normal level of 70S ribosomes but only a low rate of cp protein synthesis with spectinomycin present. In both mutants there is little accumulation of the large subunit of ribulose 1,5-bisphosphate carboxylase (Rubisco LSU), but near wild-type levels of cp synthesized r-proteins. In cells pulse-labelled with /sup 35/SO/sub 4/ and immunoprecipitated with specific antisera, the ratio of the rate of synthesis of cp r-proteins to that of Rubisco LSU is 7 times greater in both mutants than in wild-type. No difference in the rate of turnover between r-proteins and Rubisco LSU in mutant and wild-type cells was observed during a one hour chase. The mRNA levels for r-protein L1 and Rubisco LSU actually increase slightly in the mutants. These data suggest that C. reinhardtii has a translation mechanism for preferential synthesis of cp r-proteins that operates under conditions of reduced total cp protein synthesis.
- Research Organization:
- Duke Univ., Durham, NC
- OSTI ID:
- 6077873
- Report Number(s):
- CONF-8707108-
- Journal Information:
- Plant Physiol., Suppl.; (United States), Journal Name: Plant Physiol., Suppl.; (United States) Vol. 83:4; ISSN PPYSA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ALGAE
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
CARBON-CARBON LYASES
CARBOXY-LYASES
CELL CONSTITUENTS
CHLAMYDOMONAS
CHLOROPLASTS
DAYS LIVING RADIOISOTOPES
DRUGS
ENZYMES
EVEN-ODD NUCLEI
IMMUNOASSAY
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LIGHT NUCLEI
LYASES
MESSENGER-RNA
MICROORGANISMS
MUTANTS
NUCLEI
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
OXYGEN COMPOUNDS
PLANTS
PRECIPITATION
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RIBOSOMES
RIBULOSE DIPHOSPHATE CARBOXYLASE
RNA
SEPARATION PROCESSES
SULFATES
SULFUR 35
SULFUR COMPOUNDS
SULFUR ISOTOPES
SYNTHESIS
TRACER TECHNIQUES
UNICELLULAR ALGAE