Complementation of a Rhodopseudomonas sphaeroides ribulose bisphosphate carboxylase-oxygenase regulatory mutant from a genomic library

Journal Article · Tue Oct 01 00:00:00 EDT 1985 · J. Bacteriol.; (United States)

OSTI ID:5459807

Weaver, K E; Tabita, F R

A genomic library containing HindIII partial digests of Rhodopseudomonas sphaeroides HR DNA was constructed in the broad-host-range cosmid cloning vector pVK102. With a portion of this library as donor in complementation studies with the ribulose 1,5-bisphosphate carboxylase-oxygenase regulatory mutant R. sphaeroides KW25/11, a fragment of DNA which is capable of partially complementing this mutant was isolated. In four independent matings, Aut/sup +/ transconjugants which contained a hybrid plasmid carrying the same 28-kilobase-pair insert were isolated. While complemented strains were capable of growing at rates equal to that of the wild type under photoautotrophic conditions, they were not able to match wild-type levels of ribulose 1,5-biphosphate carboxylase-oxygenase activity or of form I ribulose 1,5-bisphosphate carboxylase-oxygenase protein. In addition, there is some indication that recombination may be necessary for optimal complementation to occur. The size of the complementing fragment was further reduced to 2.7 kilobase pairs by using vectors constructed for subcloning.

• Research Organization: Univ. of Texas, Austin
• OSTI ID: 5459807
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 164:1; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

550400 -- Genetics
550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BIOLOGY
CARBON-CARBON LYASES
CARBOXY-LYASES
CARBOXYLASE
DNA
ENZYME ACTIVITY
ENZYMES
GENE RECOMBINATION
GENETICS
LYASES
MICROORGANISMS
MUTANTS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
OXYGENASES
PHOTOSYNTHETIC BACTERIA
RHODOPSEUDOMONAS