In-111 tropolone complex for study of lymphocyte kinetics: Evidence for an induced defect in structure, function and viability

Conference · Sun Jan 01 04:00:00 EST 1984 · J. Nucl. Med.; (United States)

OSTI ID:6520570

Balaban, E; Simon, T R; Kulkarni, P; White, J; Newton, M; Frenkel, E

The lipid soluble In-111 and tropolone complex (In-T) has been proposed as a desirable cell labeling moiety for in vivo studies. Its advantages over In-111 complexed to oxy/sup -/ or acetylacetonate are water solubility and efficient cell labeling in plasma. The authors examined the effect of In-T on lymphocyte integrity and function in preparation for studies of lymphocyte kinetics in traffic. At equal concentrations, both normal and lymphocytes from patients with chronic lymphocytic leukemia had cellular In-T uptake consistently 20% greater than that achieved with In-111 oxine. This desirable uptake led to studies of function and viability. Lymphocyte mitogenmediated blastogenic capability (an intrinsic lymphocyte function) was measured in vitro in ficoll-hypaque isolated normal lymphocytes with varying concentrations and intervals of exposure of In-T. Marked impairment of lymphocyte blastogenic responsiveness was seen with 3 different mitogens (concanavalin A, phytohemmagglutinin P, and pokeweed mitogen). Severe functional impairment was seen when cells were exposed to a In-T concentration of 10 ..mu..l/ml for 20 minutes; and a lesser effect was noted even at 10-minute incubation exposure. Cell viability, evaluated by trypan blue exclusion, was normal immediately following cell labeling, but rapidly and progressively failed to exclude (i.e. effective viability). Scanning electron microscopy demonstrated loss of the normal surface villous architecture within 36 hours of in vitro incubation following a 20-minute exposure. Thus, although In-T has attractive features, its effect on lymphocyte structure, function and viability eliminate it for in vivo studies in traffic kinetics.

• Research Organization: Dallas VA Med. Ctr., Dallas, TX
• OSTI ID: 6520570
• Report Number(s): CONF-840619-
• Conference Information: Journal Name: J. Nucl. Med.; (United States) Journal Volume: 25:5
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD CHEMISTRY
BLOOD CIRCULATION
BODY FLUIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL PREPARATION
CHEMISTRY
COMPLEXES
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISEASES
DRUGS
EFFICIENCY
ELECTRON CAPTURE RADIOISOTOPES
FUNCTIONS
HEMIC DISEASES
IN VITRO
IN VIVO
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LABELLING
LEUKEMIA
LEUKOCYTES
LYMPHOCYTES
MATERIALS
MEMBRANES
MINUTES LIVING RADIOISOTOPES
MORPHOLOGICAL CHANGES
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
RADIOISOTOPES
RADIOPHARMACEUTICALS
SOMATIC CELLS
SYNTHESIS
TOXICITY
ULTRASTRUCTURAL CHANGES