Halogenated hydrocarbon and hydroperoxide induced lipid peroxidation in rate tissue slices

Journal Article · Thu Feb 01 00:00:00 EST 1990 · Journal of Agricultural and Food Chemistry; (USA)

DOI:https://doi.org/10.1021/jf00092a022· OSTI ID:6520390

Sano, Mitsuaki; Tappel, A L ^[1]

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1. Univ. of California, Davis (USA)

Damage to biological systems exposed simultaneously to more that one toxicant may be amplified over damage produced by individual toxicants. Thiobarbituric acid reactive substances (TBARS) were measured in rat tissue slices to investigate lipid peroxidation induced by halogenated hydrocarbons (HHC), tert-butyl hydroperoxide (BHP), or a combination of the two types of toxicant. TBARS production by liver slices incubated with 1 mM HHC occurred in the following order: BrCCL{sub 3} > CCl{sub 4} = C{sub 2}H{sub 2}Br{sub 2} > C{sub 2}H{sub 4}Cl{sub 2}; 1 mM C{sub 2}H{sub 2}Br{sub 4}, C{sub 2}H{sub 2}Cl{sub 4}, CH{sub 2}Br{sub 2}, CH{sub 2}Cl{sub 2} or C{sub 2}HBrClF{sub 3} or 0.05 mM BHP did not increase TBARS production. TBARS production by liver slices incubated with BHP was synergistic in combination with BrCCl{sub 3}, CCl{sub 4}, C{sub 2}H{sub 4}Br{sub 2}, C{sub 2}H{sub 4}Cl{sub 2}, C{sub 2}H{sub 2}Br{sub 4}, C{sub 2}H{sub 2}Cl{sub 4}, or C{sub 2}HBrClF{sub 3}, depending upon concentrations used. A synergistic effect of BHP and BrCCl{sub 3} was shown in heart slices, but not in kidney, testes, lung, or spleen slices. Synergism between the HHC and BHP was dependent upon the HHC, oxidant concentrations, and the individual tissue. This dependency may reflect the mechanisms by which these compounds are metabolized in the individual tissues as well as other tissue variables that affect lipid peroxidation.

• OSTI ID: 6520390
• Journal Information: Journal of Agricultural and Food Chemistry; (USA), Vol. 38:2; ISSN 0021-8561
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
HYDROCARBONS
TOXICITY
HYDROGEN PEROXIDE
LIPIDS
OXIDATION
ORGANIC HALOGEN COMPOUNDS
ANIMAL TISSUES
HEART
METABOLISM
RATS
SYNERGISM
ANIMALS
BODY
CARDIOVASCULAR SYSTEM
CHEMICAL REACTIONS
HYDROGEN COMPOUNDS
MAMMALS
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PEROXIDES
RODENTS
TISSUES
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology