Peroxidation in intestinal mucosa of normal and iron-overloaded rats differing in selenium status

Conference · Fri Mar 15 04:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5213196

Mahoney, A W; Vega, S ^[1]

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1. Utah State Univ., Logan (United States)

Material in the digesta may lead to lipid peroxidation of the intestinal mucosa. To study the effect of Se deficiency ({minus}Se) and Fe overload (++Fe) on mucosal free radical damage, 60 220g rats in four groups were fed torula yeast diet for 20d. Fe-overload was caused in two groups by three IM injections of Fe dextran given on days 4, 9, and 14. Fe-control rats (+Fe) were sham-injected with n-saline. Se-control rats (+Se) were given Na{sub 2}SeO{sub 3}-supplemented drinking water. Se deficiency reduced liver and mucosal glutathione peroxidase activity, but Fe overload did not. Serum, liver and mucosal Fe was higher in the Fe-overloaded rats. Fe and Se treatments did not affect hemoglobin level, but Fe-overload reduced weight gain. Fe overload increased liver and mucosal thiobarbituric acid reactive substances (TBARS), but Se status did not affect them. On days 5-9, CBrCL{sub 3}, an environmental pollutant and lipid peroxidation initiator which must be activated by cytochrome P-450, was gavaged in 10 rats from each group; the higher dose increased mucosal TBARS in Fe-overloaded rats but not Se-deficient ones. But, the lower CBrCL{sub 3} dose did not affect mucosal TBARS. Liver TBARS was not affected by CBrCL{sub 3}; however, the highest liver and mucosal TBARS levels occurred in the {minus}Se++Fe rats given the higher CBrCL{sub 3} dose. Liver cytochrome P-450 activity was not affected by Fe{minus} nor Se status.

• OSTI ID: 5213196
• Report Number(s): CONF-9104107--
• Conference Information: Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 5:5
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
BODY
DIGESTIVE SYSTEM
ELEMENTS
ENZYME ACTIVITY
ENZYMES
GASTROINTESTINAL TRACT
INTESTINES
IRON
LIPIDS
MAMMALS
MEMBRANES
METABOLISM
METALS
MIXED-FUNCTION OXIDASES
MUCOUS MEMBRANES
NUTRITIONAL DEFICIENCY
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
OXYGENASES
PROTEINS
RADICALS
RATS
RODENTS
SELENIUM
SEMIMETALS
TOXICITY
TRANSITION ELEMENTS
VERTEBRATES