Influence of environmental factors on the physiological condition and hepatic ethoxyresorufin O-deethylase (EROD) activity of gizzard shad (Dorosoma cepedianum)

Journal Article · Sat Dec 31 23:00:00 EST 1994 · Environmental Toxicology and Chemistry; (United States)

DOI:https://doi.org/10.1002/etc.5620140114· OSTI ID:6519965

Levine, S L; Oris, J T; Wissing, T E ^[1]

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1. Miami Univ., Oxford, OH (United States). Dept. of Zoology

Field and laboratory experiments were conducted to monitor seasonal cytochrome P4501A monooxygenase (MO) activity (measured as ethoxyresorufin O-deethylase (EROD) activity) in gizzard shad (Dorosoma cepedianum). Basal EROD activity declined in midsummer but returned to postspawning spring levels by late summer. Seasonal variation in basal EROD activity corresponded with changes in liver somatic index, condition factor, and percent body lipid, indicating that seasonal variation in basal field EROD activity may result from changing physiological condition. Benzo[a]pyrene induction experiments demonstrated that, although the level of EROD activity changed seasonally, the magnitude of induction relative to corn-oil-injected controls remained the same. Kinetic studies indicated monthly changes in apparent V[sub max] but no change in apparent K[sub m]. Taken together, these results suggest that seasonal changes in EROD activity reflect changes in hepatic P4501A protein concentration. This study has demonstrated that it can be inappropriate to interpret EROD activity from a suspect site without first understanding how seasonal field conditions influence P4501A activity.

• OSTI ID: 6519965
• Journal Information: Environmental Toxicology and Chemistry; (United States), Journal Name: Environmental Toxicology and Chemistry; (United States) Vol. 14:1; ISSN 0730-7268; ISSN ETOCDK
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AQUATIC ORGANISMS
AROMATICS
BENZOPYRENE
BIOLOGICAL EFFECTS
BODY
CONDENSED AROMATICS
DIGESTIVE SYSTEM
ENVIRONMENTAL EFFECTS
ENZYME ACTIVITY
ENZYMES
FISHES
GLANDS
HYDROCARBONS
LIVER
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
POLLUTION
PROTEINS
SEASONAL VARIATIONS
SENSITIVITY
VARIATIONS
VERTEBRATES
WATER POLLUTION