Influence of toxicological and environmental factors on P4501A expression in gizzard shad: Consequences for biomarker interpretation
- Miami Univ., Oxford, OH (United States). Dept. of Zoology
Laboratory and field experiments were conducted to examine the influence of selected endogenous factors on gizzard shad (Dorosoma cepedianum) P4501A expression. The endogenous factors that were examined included the influence of exposure to a P4501A inducer, the combined effect of coexposure to a P4501A inducer and a P4501A inhibitor and the influence of nutritional status on P4501A expression. Shad exposed at 16 C exhibited a dose-response and time-course for P4501A induction following waterborne benzo[a]pyrene (BaP) exposures. P4501A expression was increased by waterborne BaP concentrations at half of the BaP water solubility within 48 h and was increased by waterborne BaP concentrations at the water solubility within 6 h. Correlations between the amount of BaP removed from the water by the fish and the degree of induction were established using a one-compartment linear clearance model. Shad pretreated with the inhibitor bioconcentrated more parent-BaP compared to groups exposed to BaP alone. Postspawning shad exhibited seasonal variation in basal hepatic P4501A expression and this variation corresponded with changes in liver somatic index, condition factor, and percent body lipid, indicating that seasonal variation in basal field P4501A expression may result from seasonally changing nutritional status. Preliminary results from BaP induction and enzyme kinetics studies suggest that seasonal changes in P4501A expression may reflect changes in P4501A protein concentration.
- OSTI ID:
- 460487
- Report Number(s):
- CONF-961149--
- Country of Publication:
- United States
- Language:
- English
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