Adenylate cyclase regulation in the spermatogenic cell plasma membrane: Modulating effects of TPA and TCDD
Thesis/Dissertation
·
OSTI ID:6516805
This research was designed to compare the effects of TPA, a phorbol ester, and TCDD in a spermatogenic cell population, a target of TCDD toxicity. Membrane-bound adenylate cyclase activity was used an index of membrane function, and was quantified by the amount of {sup 32}P-cAMP formed from {sup 32}P-ATP following chromatographic separation. Exposure to male germ cells in-vitro to TPA and TCDD followed by direct measurement of enzyme activity was used to investigate the potential of each agent to perturb membrane function. TPA and TCDD consistently inhibited adenylate cyclase activity at the levels of G{sub s}-catalytic unit coupling and hormone-receptor activation, as measured by the stimulation of enzyme activity by concomitant addition of forskolin and GTP and FSH and GTP, respectively. The effect on coupling required at least 60 minutes of exposure to TPA or TCDD. Concentration-response curves demonstrated a progressive desensitization with increasing TPA concentration, while TCDD exhibited consistent inhibition over the same concentration range.
- Research Organization:
- Philadelphia Coll. of Pharmacy and Science, PA (USA)
- OSTI ID:
- 6516805
- Country of Publication:
- United States
- Language:
- English
Similar Records
Regulation of follitropin-sensitive adenylate cyclase by stimulatory and inhibitory forms of the guanine nucleotide regulatory protein in immature rat Sertoli cells
Alkaline phosphatase relieves desensitization of adenylate cyclase-coupled beta-adrenergic receptors in avian erythrocyte membranes
Heterologous desensitization of adenylate cyclase from pigeon erythrocytes under the action of the catalytic subunit of cAMP-dependent protein kinase
Thesis/Dissertation
·
Wed Dec 31 23:00:00 EST 1986
·
OSTI ID:5518283
Alkaline phosphatase relieves desensitization of adenylate cyclase-coupled beta-adrenergic receptors in avian erythrocyte membranes
Conference
·
Fri May 01 00:00:00 EDT 1987
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
·
OSTI ID:6120078
Heterologous desensitization of adenylate cyclase from pigeon erythrocytes under the action of the catalytic subunit of cAMP-dependent protein kinase
Journal Article
·
Fri Sep 20 00:00:00 EDT 1985
· Biochemistry (Engl. Transl.); (United States)
·
OSTI ID:5722391
Related Subjects
550501 -- Metabolism-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMP
ATP
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARCINOGENS
CELL CONSTITUENTS
CELL MEMBRANES
CHROMATOGRAPHY
COMPARATIVE EVALUATIONS
CYCLASES
DAYS LIVING RADIOISOTOPES
DIOXIN
DOSE-RESPONSE RELATIONSHIPS
ENZYME ACTIVITY
ENZYMES
ESTERS
GAMETES
GERM CELLS
HETEROCYCLIC COMPOUNDS
INHIBITION
ISOTOPES
LIGHT NUCLEI
LYASES
MEMBRANES
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PHORBOL ESTERS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
RADIOISOTOPES
SENSITIVITY
SEPARATION PROCESSES
SPERMATOGONIA
TOXICITY
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMP
ATP
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARCINOGENS
CELL CONSTITUENTS
CELL MEMBRANES
CHROMATOGRAPHY
COMPARATIVE EVALUATIONS
CYCLASES
DAYS LIVING RADIOISOTOPES
DIOXIN
DOSE-RESPONSE RELATIONSHIPS
ENZYME ACTIVITY
ENZYMES
ESTERS
GAMETES
GERM CELLS
HETEROCYCLIC COMPOUNDS
INHIBITION
ISOTOPES
LIGHT NUCLEI
LYASES
MEMBRANES
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PHORBOL ESTERS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
RADIOISOTOPES
SENSITIVITY
SEPARATION PROCESSES
SPERMATOGONIA
TOXICITY