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Heterologous desensitization of adenylate cyclase from pigeon erythrocytes under the action of the catalytic subunit of cAMP-dependent protein kinase

Journal Article · · Biochemistry (Engl. Transl.); (United States)
OSTI ID:5722391
; ;
Preincubation of the plasma membranes from pigeon erythrocytes with the catalytic subunit of cAMP-dependent protein kinase leads to desensitization of adenylate cyclase of the erythrocytes. The adenylate cyclase activity, measured in the presence of 10 ..mu..M isoproterenol and 50 ..mu..M GTP-..gamma..-S, is decreased by 40% in 10 min of incubation, while the activity in the presence of 50 ..mu..M GTP-..gamma..-S is decreased by 35% in 20 min. The decrease in the adenylate cyclase activity is due to an increase in the lag phase of activation of the enzyme in the presence of a GTP analog stable to hydrolysis and a decrease in the activity in the steady-state phase of activation. Heterologous desensitization of adenylate cyclase under the action of cAMP-dependent protein kinase is coupled with a decrease in the number of ..beta..-adrenoreceptors capable of passing into a state of high affinity for antagonists in the absence of guanylic nucleotides. The influence of the catalytic subunit on adenylate cyclase entirely models the process of desensitization of the enzyme absorbed in the influence of isoproterenol or cAMP on erythrocytes.
Research Organization:
M.V. Lomonosov Moscow State Univ., USSR
OSTI ID:
5722391
Journal Information:
Biochemistry (Engl. Transl.); (United States), Journal Name: Biochemistry (Engl. Transl.); (United States) Vol. 50:3; ISSN BIORA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
550301 -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMP
ANIMALS
ATP
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIRDS
BLOOD
BLOOD CELLS
BODY FLUIDS
CATALYTIC EFFECTS
CELL CONSTITUENTS
CELL MEMBRANES
CYCLASES
DEACTIVATION
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
ERYTHROCYTES
INCUBATION
ISOTOPE APPLICATIONS
LYASES
MATERIALS
MEMBRANE PROTEINS
MEMBRANES
NUCLEOTIDES
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PIGEONS
PROTEINS
RADIOENZYMATIC ASSAY
RADIORECEPTOR ASSAY
RECEPTORS
TRACER TECHNIQUES
TRANSFERASES
VERTEBRATES