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Functional changes in the properties of the. beta. -adrenoreceptors of pigeon erythrocytes under the action of the catalytic subunit of cAMP-dependent protein kinase

Journal Article · · Biochemistry (Engl. Transl.); (United States)
OSTI ID:5933451
; ;

The ..beta..-adrenoreceptors were solubilized from the plasma membranes of pigeon erythrocytes, treated with N-ethylmaleimide, using deoxycholate. The removal of the deoxycholate leads to incorporation of receptors into phospholipid vesicles and a restoration of their biological activity. After fusion of vesicles containing reconstituted receptors with vesicles containing the N/sub s/-protein and the catalytic component, a restoration of the hormonal activity of the enzyme was observed. If vesicles containing ..beta..-adrenoreceptors were incubated before fusion with the catalytic subunit of cAMP-dependent protein kinase, the hormonal activity of the preparation obtained was lowered by 45-50%. The decrease in activity occurred on account of an increase in the lag phase of activation of the enzyme in the presence of isoproterenol and GPP(NH)p, as well as on account of a decrease in the activity in the stationary phase of activation. Phosphorylation of the ..beta..-adrenoreceptors leads to a decrease in the content of the ternary isoproterenol-receptor-N/sub s/-protein complex, participating in the activation of adenylate cyclase. Thus, phosphorylation of the receptors leads to disruptions of the mechanism of transmission of the hormonal signal, analogous to those observed in the desensitization of adenylate cyclase.

Research Organization:
M.V. Lomonosov Moscow State Univ., USSR
OSTI ID:
5933451
Journal Information:
Biochemistry (Engl. Transl.); (United States), Journal Name: Biochemistry (Engl. Transl.); (United States) Vol. 50:9; ISSN BIORA
Country of Publication:
United States
Language:
English

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Related Subjects

550201 -- Biochemistry-- Tracer Techniques
550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMP
ANIMALS
BIOCHEMISTRY
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BIRDS
BLOOD
BLOOD CELLS
BODY FLUIDS
CATALYTIC EFFECTS
CHEMICAL REACTIONS
CHEMISTRY
CYCLASES
ENZYME ACTIVITY
ENZYMES
ERYTHROCYTES
ESTERS
FUNCTIONS
HORMONES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
LYASES
MATERIALS
MEMBRANE PROTEINS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
PIGEONS
PROTEINS
RADIORECEPTOR ASSAY
RECEPTORS
STRUCTURE-ACTIVITY RELATIONSHIPS
SYNTHESIS
TRACER TECHNIQUES
TRANSFERASES
TRITIUM COMPOUNDS
VERTEBRATES