Effect of the hydrolytic state of dietary protein on post-irradiation morbidity and mucosal cell regeneration

Journal Article · Sun Mar 01 04:00:00 EST 1987 · Int. J. Radiat. Oncol., Biol. Phys.; (United States)

DOI:https://doi.org/10.1016/0360-3016(87)90013-7· OSTI ID:6515721

Beitler, M K; Mahler, P A; Yamanaka, W K; Guy, D G; Hutchinson, M L

Diets containing hydrolyzed casein have been observed to enhance post-irradiation intestinal mucosal recovery. The intake and the composition of such diets were not carefully controlled. This study attempted to do so. Male specific pathogen-free Sprague-Dawley rats were randomized to receive either an enzymatically hydrolyzed casein semi-purified diet (EHC), a whole casein semi-purified diet (WC), or powdered lab chow (C). All diets were isonitrogenous, and the WC and C rats were pair-fed to the ad libitum fed EHC rats. Seven days after initiation of feeding, the rats were abdominally irradiated with a single 9.0 Gy dose of 137Cs gamma rays. The rats were continued on the diets for another 5 days. Intestinal mucosa from transverse segments at the duodenum, jejunum, proximal ileum, and distal ileum were measured for incorporation of (/sup 3/H methyl) thymidine 1 hour after intraperitoneal injection. Incorporation reached a maximum by day 4 post-irradiation regardless of diet or segment. Incorporation in the duodenum was enhanced by the EHC diet compared to the C diet, while the incorporation in the jejunum was initially suppressed by the EHC diet compared to the WC diet. In the jejunum, the number of mitoses per crypt of 25 anti-mesenteric crypts post-irradiation was increased by the EHC diet. Prior to irradiation, all groups gained similar amounts of weight. After irradiation, the C rats lost weight, while the EHC and WC rats remained the same or gained weight. Guaiac tests for occult blood were negative prior to irradiation, but positive for all rats on days 1-5 postirradiation. When calorie and protein intakes were controlled, different areas of the small intestine responded differently to EHC.

• Research Organization: Univ. of Washington, Seattle
• OSTI ID: 6515721
• Journal Information: Int. J. Radiat. Oncol., Biol. Phys.; (United States), Journal Name: Int. J. Radiat. Oncol., Biol. Phys.; (United States) Vol. 3; ISSN IOBPD
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ABDOMEN
ALKALI METAL ISOTOPES
ANIMALS
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
BODY AREAS
CASEIN
CESIUM 137
CESIUM ISOTOPES
DIET
DIGESTIVE SYSTEM
EXTERNAL IRRADIATION
GASTROINTESTINAL TRACT
HETEROCYCLIC COMPOUNDS
INJURIES
INTESTINES
IRRADIATION
ISOTOPES
MAMMALS
MEMBRANES
MUCOUS MEMBRANES
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PROTEINS
PYRIMIDINES
RADIATION EFFECTS
RADIATION INJURIES
RADIOISOTOPES
RATS
REGENERATION
RESPONSE MODIFYING FACTORS
RIBOSIDES
RODENTS
SMALL INTESTINE
THYMIDINE
VERTEBRATES
YEARS LIVING RADIOISOTOPES