Skip to main content
U.S. Department of Energy
Office of Scientific and Technical Information

Effects of NO/sub 2/ on immune responses in pulmonary lymph of sheep

Journal Article · · J. Toxicol. Environ. Health; (United States)
Sheep in which the efferent duct of the caudal mediastinal lymph node was cannulated were exposed to 5 ppm NO/sub 2/, 1.5 h/d for 10 or 11 d. Immune responses were assessed by measuring the daily output of hemolytic plaque-forming cells (PFC) in pulmonary lymph, following intrabronchial immunization with horse red blood cells (HRBC) and phytohemagglutinin- (PHA) induced transformation of blood and pulmonary lymph lymphocytes. Sheep immunized 2 d after termination of NO/sub 2/ exposure had reduced outputs of PFC as compared to those seen in sheep challenged 4 d after NO/sub 2/ exposure. Animals immunized 4 d after NO/sub 2/ exposure had outputs similar to those of air control sheep.A reduction of 38-87% in the transformation index of both blood and pulmonary lymph lymphocytes was observed in sheep exposed to NO/sub 2/. These results suggest that intermittent, short-term exposure to 5 ppm NO/sub 2/ may temporarily alter pulmonary immune responsiveness.
Research Organization:
Brookhaven National Lab., Upton, NY
DOE Contract Number:
AC02-76CH00016
OSTI ID:
6513437
Journal Information:
J. Toxicol. Environ. Health; (United States), Journal Name: J. Toxicol. Environ. Health; (United States) Vol. 10:2; ISSN JTEHD
Country of Publication:
United States
Language:
English