Pulmonary mononuclear cells: studies of pulmonary lymph and bronchoalveolar cells of sheep

Journal Article · Sun Feb 01 04:00:00 EST 1981 · RES, J. Reticuloendothel. Soc.; (United States)

OSTI ID:5177248

Chanana, A D; Chandra, P; Joel, D D

Access to lung lymph was obtained by cannulation of the efferent lymph duct of the caudal mediastinal lymph node of sheep. Bronchoalveolar cells were obtained by subsegmental lavages. Bronchoalveolar lymphocytes were purified using nylon wool columns. T and B lymphocytes were identified by indirect and direct immunofluorescence, respectively. The functional potential of pulmonary and blood lymphocytes was determined by the proliferative responses to phytohemagglutinin. Macrophages were the predominant cell type in sheep bronchoalveolar air spaces. T lymphocytes constituted the major fraction of blood, lung lymph and bronchoalveolar lymphocytes. Repeated bronchoalveolar lavages did not cause any apparent change in lavage cell populations. The response of lymphocytes in lung lymph and blood to PHA was comparable. Purified lavage lymphocytes exhibited a high degree of spontaneous activation in culture.

• Research Organization: Brookhaven National Lab., Upton, NY
• DOE Contract Number: AC02-76CH00016
• OSTI ID: 5177248
• Journal Information: RES, J. Reticuloendothel. Soc.; (United States), Journal Name: RES, J. Reticuloendothel. Soc.; (United States) Vol. 29:2; ISSN RESJA
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
AGGLUTININS
ANIMAL CELLS
ANIMALS
ANTIBODIES
BIOLOGICAL MATERIALS
BIOLOGY
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBOHYDRATES
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
CYTOLOGY
DOMESTIC ANIMALS
HEMAGGLUTININS
LAVAGE
LEUKOCYTES
LUNGS
LYMPH
LYMPHOCYTES
MACROPHAGES
MAMMALS
MATERIALS
MITOGENS
MUCOPROTEINS
ORGANIC COMPOUNDS
ORGANS
PHAGOCYTES
PHYTOHEMAGGLUTININ
POLYSACCHARIDES
PROTEINS
RESPIRATORY SYSTEM
RUMINANTS
SACCHARIDES
SHEEP
SOMATIC CELLS
VERTEBRATES