Synergy between the NF-E1 erythroid-specific transcription factor and the CACCC factor in the erythroid-specific promoter of the human prophobilinogen deaminase gene

Journal Article · Sun Jul 01 00:00:00 EDT 1990 · Molecular and Cellular Biology; (USA)

DOI:https://doi.org/10.1128/MCB.10.7.3838· OSTI ID:6511372

Frampton, J ^[1]; Walker, M; Plumb, M; Harrison, P R ^[2]

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1. European Molecular Biology Lab., Heidelberg (Germany, F.R.)
2. Cancer Research Campaign Beatson Lab., Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD (GB)

This paper reports that a 114-base-pair promoter fragment of the human porphobilinogen deaminase gene functioned in an erythroid-specific manner in transient transfection experiments. Site-directed mutagenesis of the binding site for the erythroid-specific transcription factor (NF-E1) or an adjacent CACCC motif abolished the promoter activity. Increasing the spacing between these sites progressively reduced promoter activity, but there was no evidence that a critical alignment of the two factors on the DNA helix was required.

• OSTI ID: 6511372
• Journal Information: Molecular and Cellular Biology; (USA), Journal Name: Molecular and Cellular Biology; (USA) Vol. 10:7; ISSN MCEBD; ISSN 0270-7306
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
DEAMINATION
ENZYME ACTIVITY
ERYTHROCYTES
FUNCTIONS
GENE REGULATION
GENE REPRESSORS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
MATERIALS
NUCLEOPROTEINS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PORPHYRINS
PROTEINS
TRANSCRIPTION FACTORS