Strand breaks and alkali-labile bonds induced by ultraviolet light in DNA with 5-bromouracil in vivo. [Escherichia coli]
Journal Article
·
· Biophys. J.; (United States)
Supercircular lambda phage DNA with 10 bromouracils/100 thymine bases, irradiated with 313 nm light in Tris buffer and sedimented on alkaline and neutral gradients, showed 4.6 alkali-labile bonds per true single-strand break, in agreement with Hewitt and Marburger (1975 Photochem. Photobiol. 21:413). The same DNA irradiated in Escherichia coli host cells showed about the same number of breaks in alkaline gradients for equal fluence, but only 0.5 alkali-labile bond per true break. Similarly, E. coli DNA with bromouracil irradiated in the cells showed only 10 to 20% more breaks when denatured with 0.1 M NaOH than under neutral conditions with 9 M sodium perchlorate at 50/sup 0/C. These results show that true single-strand breaks occur more frequently than alkali-labile bonds after ultraviolet irradiation of DNA containing bromouracil in cells.
- Research Organization:
- Yale Univ., New Haven, CT.
- OSTI ID:
- 6504166
- Journal Information:
- Biophys. J.; (United States), Journal Name: Biophys. J.; (United States) Vol. 24:3; ISSN BIOJA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Double-strand breaks from single photochemical events in DNA containing 5-bromouracil. [Escherichia coli; uv radiation]
Biopsy measurement of DNA damage and repair in vivo: single-strand breaks, alkali-labile bonds, and endonuclease-sensitive sites
STUDIES OF ULTRAVIOLET IRRADIATION OF ESCHERICHIA COLI CONTAINING 5- BROMOURACIL IN ITS DNA
Journal Article
·
Thu Nov 30 23:00:00 EST 1978
· Biophys. J.; (United States)
·
OSTI ID:6469972
Biopsy measurement of DNA damage and repair in vivo: single-strand breaks, alkali-labile bonds, and endonuclease-sensitive sites
Journal Article
·
Thu Jul 01 00:00:00 EDT 1982
· Radiat. Res.; (United States)
·
OSTI ID:6707886
STUDIES OF ULTRAVIOLET IRRADIATION OF ESCHERICHIA COLI CONTAINING 5- BROMOURACIL IN ITS DNA
Journal Article
·
Wed Jun 01 00:00:00 EDT 1960
· Journal of General Microbiology (England)
·
OSTI ID:4054852
Related Subjects
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400500 -- Photochemistry
560111 -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
560112* -- Radiation Effects on Biochemicals-- In Microorganisms-- (-1987)
560131 -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANTIMETABOLITES
ANTIMITOTIC DRUGS
AZINES
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BROMOURACILS
CHEMICAL REACTIONS
COMPARATIVE EVALUATIONS
DNA
DRUGS
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IN VITRO
IN VIVO
IRRADIATION
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOTOCHEMICAL REACTIONS
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY EFFECTS
STRAND BREAKS
ULTRAVIOLET RADIATION
URACILS
400500 -- Photochemistry
560111 -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
560112* -- Radiation Effects on Biochemicals-- In Microorganisms-- (-1987)
560131 -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANTIMETABOLITES
ANTIMITOTIC DRUGS
AZINES
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BROMOURACILS
CHEMICAL REACTIONS
COMPARATIVE EVALUATIONS
DNA
DRUGS
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IN VITRO
IN VIVO
IRRADIATION
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOTOCHEMICAL REACTIONS
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY EFFECTS
STRAND BREAKS
ULTRAVIOLET RADIATION
URACILS