Tumor neutralization, immunotherapy, and chemoimmunotherapy of a friend leukemia with cells secondarily sensitized in vitro. II. Comparison of cells cultured with and without tumor to noncultured immune cells. [X radiation]

Journal Article · Fri Dec 01 00:00:00 EST 1978 · J. Immunol.; (United States)

OSTI ID:6504131

Cheever, M A; Greenberg, P D; Fefer, A

The aim of this study was to determine if cells primed in vivo could be rendered more effective in killing tumor by secondary sensitization in vitro. C57BL/6 mice were primed with a viable antigenic syngeneic Friend leukemia (FBL-3). The immune spleen cells secondarily sensitized by 5-day culture with irradiated FBL-3 (CiFx) became significantly more effective than fresh noncultured immune cells (Ci) in in vitro cytotoxicity, tumor neutralization (Winn assay), and immunotherapy of an early leukemia. However, in chemoimmunotherapy of advanced leukemia, CiFx were no more effective than Ci. Moreover, Ci cultured with irradiated C57BL/6 spleen cells (CiCx), i.e., without secondary sensitization in vitro, became markedly less effective than noncultured Ci. C57BL/6 mice inoculated with 5 x 10/sup 6/ FBL-3 on day 0 received on day 5 cyclophosphamide (CY) plus Ci, CiFx, or CiCx. CY alone doubled the median survival time (MST) to day 26.

• Research Organization: Univ. of Washington, Seattle
• OSTI ID: 6504131
• Journal Information: J. Immunol.; (United States), Vol. 121:6
• Country of Publication: United States
• Language: English

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