Intestinal transport: studies with isolated epithelial cells

Journal Article · Fri Nov 30 23:00:00 EST 1979 · Environ. Health Perspect.; (United States)

DOI:https://doi.org/10.1289/ehp.793337· OSTI ID:6503267

Kimmich, G A

Isolated intestinal epithelial cells have been extremely useful for characterizing the nature of intestinal absorption processes and for providing insight into the energetics of Na/sup +/-dependent transport systems. This report describes a number of experimental approaches which have been used for investigating the specific epithelial transport systems involved in sugar absorption, but provides information which ultimately should prove useful for characterizing a number of different intestinal transport events. Similar experiments should also prove useful for exploring the effect of environmental agents on the function of intestinal tissue. In the case of sugars, net absorption is accomplished via a mucosal, Na/sup +/-dependent concentrative transport system acting in sequence with a passive serosal system which does not require Na/sup +/. The serosal system limits the full gradient-forming capability of the muscosal system. Agents such as phloretin or cytochalasin B which inhibit serosal transport allow the cells to establish sugar gradients as high as 70 fold in contrast to 10 to 15 fold gradients observed for control cells. Sevety-fold sugar gradients cannot be explained in terms of the energy available in the electrochemical potential for Na/sup +/ if the Na/sub 2/: sugar coupling stoichiometry is 1:1 as commonly assumed. New information indicates that the true Na/sup +/:sugar stoichiometry is in fact 2:1. Flow of two Na/sup +/ ions per sugar molecule down the transmembrane electrochemical potential for Na/sup +/ provides more than sufficient energy to account for observed 70 fold sugar gradients. If flow of sugar by other routes could be completely inhibited, theoretical sugar gradients as high as 400 could be achieved assuming that the cells maintain a membrane potential of -36 mV as measured for intact tissue.

• Research Organization: Univ. of Rochester Medical Center, NY
• OSTI ID: 6503267
• Journal Information: Environ. Health Perspect.; (United States), Journal Name: Environ. Health Perspect.; (United States) Vol. 33; ISSN EVHPA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
550301 -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ABSORPTION
ALKALI METALS
ANIMAL TISSUES
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIRDS
BODY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CELL CULTURES
CHEMISTRY
CHICKENS
DIGESTIVE SYSTEM
DISACCHARIDES
ELECTRIC POTENTIAL
ELECTROCHEMISTRY
ELEMENTS
EPITHELIUM
FOWL
GASTROINTESTINAL TRACT
INTESTINAL ABSORPTION
INTESTINES
KINETICS
LABELLED COMPOUNDS
MEMBRANE TRANSPORT
MEMBRANES
METALS
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
REACTION KINETICS
SACCHARIDES
SACCHAROSE
SODIUM
TISSUES
UPTAKE
VERTEBRATES