cap alpha. -Methylglucoside satisfies only Na/sup +/-dependent transport system of intestinal epithelium
The unidirectional influx of ..cap alpha..-methylglucoside (..cap alpha..-MG) by isolated chicken intestinal epithelial cells is 98% inhibited by phlorizin. The remaining 2% of the total influx occurs in the absence of Na/sup +/, is not sensitive to phloretin, and is equal to the diffusional entry rate for 2-deoxyglucose. The glucoside is much more strongly accumulated (75-fold) than 3-O-methylglucose (3-OMG) (10-fold). Inhibitors of the serosal sugar carrier (phloretin, cytochalasin B, theophylline, and flavanoids) do not enhance ..cap alpha..-MG accumulation. It is concluded that the glycoside is not a substrate for the intestinal serosal transport system. Steady-state gradients of the sugar can be represented accurately by a concentrative, phlorizin-sensitive system that is opposed by a diffusional efflux process.
- Research Organization:
- Univ. of Rochester Medical Center, NY
- OSTI ID:
- 5344647
- Journal Information:
- Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 241; ISSN AJPHA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Effects of phloretin and theophylline on 3-O-methylglucose transport by intestinal epithelial cells
Effects of phloretin and theophylline on 3-O-methylglucose transport by intestinal epithelial cells
Related Subjects
551000* -- Physiological Systems
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BIOLOGICAL ACCUMULATION
BIOLOGICAL EFFECTS
BIRDS
BODY
CARBOHYDRATES
CATIONS
CHARGED PARTICLES
CHICKENS
CHLORIDES
CHLORINE COMPOUNDS
DIGESTIVE SYSTEM
EPITHELIUM
FOWL
GASTROINTESTINAL TRACT
GLYCOSIDES
HALIDES
HALOGEN COMPOUNDS
INHIBITION
INTESTINES
IONS
KETONES
MEMBRANE TRANSPORT
METABOLISM
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
PHLORIZIN
SACCHARIDES
SODIUM CHLORIDES
SODIUM COMPOUNDS
TISSUES
VERTEBRATES