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Pyrimidine biosynthesis in normal and transformed cells

Journal Article · · J. Supramol. Struct.; (United States)
;

We have developed procedures for sensitive measurement of specific radioactivities of pyrimidine nucleosides excreted from cells in culture. The changes in the observed values reflect dilution of the added isotope through de novo biosynthesis of nonradioactive pyrimidine nucleotides or by shifting and equilibration of other nucleotide pools into the free uridine pool. It is thus possible to monitor uridine biosynthesis occurring in intact cells without destroying or disrupting the cell population. On comparing a series of normal and transformed lines, we have observed several growth-dependent patterns of change in specific activity and levels of uridine excretion and the temporal appearance of these changes.

Research Organization:
Oak Ridge National Lab., TN
DOE Contract Number:
W-7405-ENG-26
OSTI ID:
6503125
Journal Information:
J. Supramol. Struct.; (United States), Journal Name: J. Supramol. Struct.; (United States) Vol. 11; ISSN JSPMA
Country of Publication:
United States
Language:
English

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Related Subjects

550301* -- Cytology-- Tracer Techniques
550501 -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
CARBON 14
CARBON ISOTOPES
CELL CULTURES
CELL CYCLE
CLEARANCE
COMPARATIVE EVALUATIONS
CONNECTIVE TISSUE CELLS
EVEN-EVEN NUCLEI
EXCRETION
FIBROBLASTS
HAMSTERS
HETEROCYCLIC COMPOUNDS
HYDROGEN ISOTOPES
HYDROXY COMPOUNDS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MAMMALS
METABOLISM
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHENOTYPE
PYRIMIDINES
RADIOISOTOPES
RIBOSIDES
RODENTS
SOMATIC CELLS
SYNTHESIS
TIME DEPENDENCE
TRITIUM
URACILS
URIDINE
VERTEBRATES
YEARS LIVING RADIOISOTOPES