Pyrimidine biosynthesis in normal and transformed cells
We have developed procedures for sensitive measurement of specific radioactivities of pyrimidine nucleosides excreted from cells in culture. The changes in the observed values reflect dilution of the added isotope through de novo biosynthesis of nonradioactive pyrimidine nucleotides or by shifting and equilibration of other nucleotide pools into the free uridine pool. It is thus possible to monitor uridine biosynthesis occurring in intact cells without destroying or disrupting the cell population. On comparing a series of normal and transformed lines, we have observed several growth-dependent patterns of change in specific activity and levels of uridine excretion and the temporal appearance of these changes.
- Research Organization:
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 6503125
- Journal Information:
- J. Supramol. Struct.; (United States), Vol. 11
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CELL CULTURES
BIOSYNTHESIS
URIDINE
METABOLISM
CARBON 14
CELL CYCLE
COMPARATIVE EVALUATIONS
EXCRETION
FIBROBLASTS
HAMSTERS
LABELLED COMPOUNDS
PHENOTYPE
TIME DEPENDENCE
TRITIUM
ANIMAL CELLS
ANIMALS
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBON ISOTOPES
CLEARANCE
CONNECTIVE TISSUE CELLS
EVEN-EVEN NUCLEI
HETEROCYCLIC COMPOUNDS
HYDROGEN ISOTOPES
HYDROXY COMPOUNDS
ISOTOPES
LIGHT NUCLEI
MAMMALS
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RADIOISOTOPES
RIBOSIDES
RODENTS
SOMATIC CELLS
SYNTHESIS
URACILS
VERTEBRATES
YEARS LIVING RADIOISOTOPES
550301* - Cytology- Tracer Techniques
550501 - Metabolism- Tracer Techniques