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Phycobilisome-thylakoid topography on photosynthetically active vesicles of Porphyridium cruentum

Journal Article · · Plant Physiol.; (United States)
DOI:https://doi.org/10.1104/pp.67.4.608· OSTI ID:6501393
Conditions are described for isolating functional phycobilisome-thylakoid vesicles from the red alga Porphyridium cruentum. They required ferricyanide as an oxidant and had O/sub 2/ evolution rates higher than whole cells. Energy transfer to photosystem II chlorophyll was evident from a high F695 nanometer (-196 C) emission peak. In electron micrographs of negatively stained material, the active thylakoid vesicles were found covered by closely spaced phycobilisomes on their external surface. The phycobilisome number in negatively stained vesicles was 450 per square micrometer, which was in the same range as the 400 per square micrometer observed in surface sections.
Research Organization:
Smithsonian Institution, Rockville, MD
DOE Contract Number:
EY-76-S-05-4310
OSTI ID:
6501393
Journal Information:
Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 67:4; ISSN PLPHA
Country of Publication:
United States
Language:
English