Characterization of a purified photosystem II-phycobilisome particle preparation from Porphyridium cruentum

Journal Article · Tue Jan 01 04:00:00 EST 1985 · Plant Physiol.; (United States)

DOI:https://doi.org/10.1104/pp.77.3.626· OSTI ID:5944309

Chereskin, B M; Clement-Metral, J D; Gantt, E

Detergent preparations isolated from thylakoids of the red alga Porphyridium cruentum, in a sucrose, phosphate, citrate, magnesium chloride medium consist of phycobilisomes and possess high rates of photosystem II activity. Characterization of these particles shows that the O/sub 2/-evolving activity is stable for several hours and the pH optimum is about 6.5 to 7.2. Response of the system to light, electron donors and acceptors, and inhibitors verify that the observed activity, measured both as O/sub 2/ evolution and 2,6-dichlorophenol-indophenol reduction, is due to photosystem II. Furthermore, photosystem II is functionally coupled to the phycobilisome in this preparation since green light, absorbed by phycobilisomes of P. cruentum, is effective in promoting both O/sub 2/ evolution and 2,6-dichlorophenol-indophenol reduction. Photosystem II activity declines when light with wavelengths shorter than 665 nm is removed. Both 3-(3,4-dichlorophenyl)-1,1-dimethylurea and atrazine inhibit photosystem II activity in this preparation, indicating that the herbicide binding site is a component of the photosystem II-phycobilisome particle. 24 references, 4 figures, 2 tables.

• Research Organization: Smithsonian Environmental Research Center, Rockville, MD
• DOE Contract Number: AS05-76ER04310
• OSTI ID: 5944309
• Journal Information: Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 77; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

14 SOLAR ENERGY
140505 -- Solar Energy Conversion-- Photochemical
Photobiological
& Thermochemical Conversion-- (1980-)
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ALGAE
BIOCHEMICAL REACTION KINETICS
CELL CONSTITUENTS
CHEMICAL REACTIONS
ELEMENTS
HERBICIDES
KINETICS
MEMBRANE PROTEINS
NONMETALS
ORGANIC COMPOUNDS
OXYGEN
PESTICIDES
PHOTOCHEMICAL REACTIONS
PHOTOSYNTHESIS
PHOTOSYNTHETIC REACTION CENTERS
PHYCOBILISOMES
PLANTS
PROTEINS
PURIFICATION
REACTION KINETICS
RECEPTORS
SYNTHESIS