Flow cytometer measurement of binding assays

Patent · Tue May 12 04:00:00 EDT 1987

OSTI ID:6500624

Saunders, G C

A process is described for the immunochemical determination of the amount of antigen present in a sample. The process comprises: providing a known quantity of spheres with antibody coating; providing a quantity of smaller spheres with an antigen coating. The antigen is the same as the antigen present in the sample. The smaller spheres are fluorescently labeled; combining the known quantity of spheres with sample and allowing reaction between the antigen in the sample and the antibody on the spheres to occur for a set time period; and combining an excess of the smaller spheres with the mixture of the spheres and the sample produced in a preceding step and then allowing the reactions of antigen in sample and antigen on the smaller spheres with the antibody on spheres to reach equilibrium.

• Assignee: Dept. of Energy, Washington, DC
• Patent Number(s): US 4665020
• OSTI ID: 6500624
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIBODIES
ANTIGEN-ANTIBODY REACTIONS
ANTIGENS
CELL FLOW SYSTEMS
COATINGS
DISPERSIONS
FLOWMETERS
FLUORESCENCE
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
LABELLING
LUMINESCENCE
MEASURING INSTRUMENTS
METERS
MIXTURES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
SAMPLING
SPHERES
TRACER TECHNIQUES