Endogenous type C viral gene expression in cultures of fetal diploid baboon cells treated with 5'-bromodeoxyuridine
Cultures of fetal diploid baboon fibroblasts treated with 5-bromodeoxyuridine synthesized protein antigenically related to baboon endogenous type C viral gag gene product, p28. Radioimmunoassays detected p28 antigenic specificities indistinguishable from those of purified virus. However, viral RNA-dependent DNA polymerase was not detected in culture fluids, and infectious virus was rarely recovered by cocultivation with susceptible heterologous cells. Extracellular particles containing p28 were not readily detected, further indicating that viral gag gene-coded proteins were synthesized independently of whole virus. Normal cultures of the same baboon cells exhibited endogenous expression of a glycoprotein antigenically related to BEV gp70, suggesting differential regulation of the endogenous gag and env gene-coded products. Baboon cell cultures exogenously infected with BEV produced extracellular particles having viral p28 and gp70 as measured by radioimmunoassays of culture fluids. Since induced cultures have about 10% positive cells versus close to 100% for infected culture, the amount of p28 per producing cell was about the same in both cell populations.
- Research Organization:
- Oak Ridge National Lab., TN
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 6498124
- Journal Information:
- Virology; (United States), Journal Name: Virology; (United States) Vol. 110:2; ISSN VIRLA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTIMETABOLITES
APES
AZINES
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BROMOURACILS
BUDR
CARBOHYDRATES
CONNECTIVE TISSUE CELLS
DNA
DRUGS
ENZYMES
FIBROBLASTS
GENES
GENETIC EFFECTS
GLUCOPROTEINS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
MAMMALS
MICROORGANISMS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
NUCLEOTIDYLTRANSFERASES
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
PRIMATES
PROTEINS
PYRIMIDINES
RADIOASSAY
RADIOIMMUNOASSAY
RIBOSIDES
SACCHARIDES
SOMATIC CELLS
SYNTHESIS
TRACER TECHNIQUES
TRANSFERASES
URACILS
VERTEBRATES
VIRUSES