Expression of baboon endogenous virus in exogenously infected baboon cells
- Oak Ridge National Lab., TN
Strains of low-passage, fetal diploid, baboon (Papio cynocephalus) fibroblasts were susceptible to exogenous infection with three independent isolates of baboon endogenous virus, as measured by an immunofluorescence assay specific for viral p28. Infectivity of the M7 strain of baboon endogenous virus for baboon cells of fetal skin muscle origin was equivalent to that for human and dog cells in that similar, linear, single-hit titration patterns were obtained. The assay for supernatant RNA-dependent DNA polymerase, however, showed that baboon cells produced only low levels of virus after infection compared with the production by heterologous cells. The results showed that baboon endogenous virus was capable of penetrating baboon cells and that viral genes were expressed in infected cells. Replication of complete infectious virus was restricted, however, indicating that in this primate system homologous cells differentially regulated the expession of viral genes.
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 6333368
- Journal Information:
- J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 30:1; ISSN JOVIA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
APES
BIOASSAY
CONNECTIVE TISSUE CELLS
DATA
DATA FORMS
DNA
ENZYMES
EXPERIMENTAL DATA
FIBROBLASTS
GRAPHS
IMMUNE REACTIONS
INFECTIVITY
INFORMATION
ISOLATED VALUES
MAMMALS
MICROORGANISMS
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
PARASITES
POLYMERASES
PRIMATES
RNA
SOMATIC CELLS
VERTEBRATES
VIRUSES