Effect of 3-aminobenzamide on the rate of ligation during repair of alkylated DNA in human fibroblasts
Journal Article
·
· Cancer Res.; (United States)
OSTI ID:6497981
3-Aminobenzamide, an inhibitor of polyadenosine dephosphoribose polymerase, produced rapid reversible changes in single-strand break frequencies in DNA from primary human fibroblasts damaged by alkylating agents, but it did not cause such changes in the DNA of cells damaged by ultraviolet light. The increase in single-strand peak frequencies was not due to an accumulation of blocked repair sites, such as occurs with DNA polymerase inhibitors, but to a delay in the rejoining of induced breaks. 3-Aminobenzamide increases the net break frequency that results from a dynamic balance between excision and ligation. This balance appears to be regulated at the ligation step by adenosine diphosphate ribosylation, which is rapidly altered by addition or removal of 3-aminobenzamide. The rapidity with which strand break frequencies change in the presence of 3-aminobenzamide implies that individual strand breaks resulting from excision at any time after exposure have a lifetime of no more than about 30 min in the cell.
- Research Organization:
- Univ. of California, San Francisco
- DOE Contract Number:
- AC03-76SF01012
- OSTI ID:
- 6497981
- Journal Information:
- Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 43; ISSN CNREA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Effect of 3-aminobenzamide on the rate of ligation during repair of alkylated DNA in human fibroblasts
Differences in the regulation by poly(ADP-ribose) of repair of DNA damage from alkylating agents and ultraviolet light according to cell type
Inhibition of repair patch ligation by an inhibitor of poly(ADP-ribose) synthesis in normal human fibroblasts damaged with ultraviolet radiation
Journal Article
·
Fri Jul 01 00:00:00 EDT 1983
· Cancer Res.; (United States)
·
OSTI ID:5348890
Differences in the regulation by poly(ADP-ribose) of repair of DNA damage from alkylating agents and ultraviolet light according to cell type
Journal Article
·
Wed Aug 10 00:00:00 EDT 1983
· J. Biol. Chem.; (United States)
·
OSTI ID:6241557
Inhibition of repair patch ligation by an inhibitor of poly(ADP-ribose) synthesis in normal human fibroblasts damaged with ultraviolet radiation
Journal Article
·
Mon Feb 29 23:00:00 EST 1988
· Mol. Pharmacol.; (United States)
·
OSTI ID:7025290
Related Subjects
550400 -- Genetics
560306* -- Chemicals Metabolism & Toxicology-- Man-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADP
ALDEHYDES
ALKYLATING AGENTS
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CARBOHYDRATES
CONNECTIVE TISSUE CELLS
DNA
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
FIBROBLASTS
GENETIC EFFECTS
HUMAN POPULATIONS
MONOSACCHARIDES
NUCLEIC ACIDS
NUCLEOTIDES
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
PENTOSES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
POPULATIONS
RECOVERY
REPAIR
RESPONSE MODIFYING FACTORS
RIBOSE
SACCHARIDES
SOMATIC CELLS
STRAND BREAKS
TRANSFERASES
560306* -- Chemicals Metabolism & Toxicology-- Man-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADP
ALDEHYDES
ALKYLATING AGENTS
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CARBOHYDRATES
CONNECTIVE TISSUE CELLS
DNA
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
FIBROBLASTS
GENETIC EFFECTS
HUMAN POPULATIONS
MONOSACCHARIDES
NUCLEIC ACIDS
NUCLEOTIDES
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
PENTOSES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
POPULATIONS
RECOVERY
REPAIR
RESPONSE MODIFYING FACTORS
RIBOSE
SACCHARIDES
SOMATIC CELLS
STRAND BREAKS
TRANSFERASES