Effect of 3-aminobenzamide on the rate of ligation during repair of alkylated DNA in human fibroblasts
Journal Article
·
· Cancer Res.; (United States)
OSTI ID:5348890
3-Aminobenzamide, an inhibitor of polyadenosine diphosphoribose polymerase, produced rapid reversible changes in single-strand break frequencies in DNA from primary human fibroblasts damaged by alkylating agents, but it did not cause such changes in the DNA of cells damaged by ultraviolet light. The increase in single-strand peak frequencies was not due to an accumulation of blocked repair sites, such as occurs with DNA polymerase inhibitors, but to a delay in the rejoining of induced breaks. 3-Aminobenzamide increases the net break frequency that results from a dynamic balance between excision and ligation. This balance appears to be regulated at the ligation step by adenosine diphosphate ribosylation, which is rapidly altered by addition or removal of 3-aminobenzamide. The rapidity with which strand break frequencies change in the presence of 3-aminobenzamide implies that individual strand breaks resulting from excision at any time after exposure have a lifetime of no more than about 30 min in the cell.
- Research Organization:
- Laboratory of Radiobiology and Environmental Health, University of California, San Francisco
- OSTI ID:
- 5348890
- Journal Information:
- Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 43:7; ISSN CNREA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Effect of 3-aminobenzamide on the rate of ligation during repair of alkylated DNA in human fibroblasts
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Journal Article
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Fri Jul 01 00:00:00 EDT 1983
· Cancer Res.; (United States)
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OSTI ID:6497981
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Journal Article
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Wed Aug 10 00:00:00 EDT 1983
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OSTI ID:6241557
Inhibition of repair patch ligation by an inhibitor of poly(ADP-ribose) synthesis in normal human fibroblasts damaged with ultraviolet radiation
Journal Article
·
Mon Feb 29 23:00:00 EST 1988
· Mol. Pharmacol.; (United States)
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OSTI ID:7025290
Related Subjects
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATING AGENTS
AMIDES
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CULTURES
CONNECTIVE TISSUE CELLS
DNA
ELECTROMAGNETIC RADIATION
ENZYME INHIBITORS
ENZYMES
FIBROBLASTS
INJURIES
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
RADIATION EFFECTS
RADIATION INJURIES
RADIATIONS
RECOVERY
REPAIR
SOMATIC CELLS
STRAND BREAKS
TRANSFERASES
ULTRAVIOLET RADIATION
560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATING AGENTS
AMIDES
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CULTURES
CONNECTIVE TISSUE CELLS
DNA
ELECTROMAGNETIC RADIATION
ENZYME INHIBITORS
ENZYMES
FIBROBLASTS
INJURIES
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
RADIATION EFFECTS
RADIATION INJURIES
RADIATIONS
RECOVERY
REPAIR
SOMATIC CELLS
STRAND BREAKS
TRANSFERASES
ULTRAVIOLET RADIATION