Molecular analyses of in vivo hprt mutant T cells from atomic bomb survivors
In vivo-derived hprt-deficient mutant T cells isolated from three nonirradiated controls and two atomic bomb survivors were studied by Southern blot analysis to investigate the molecular spectra of the mutations. Mutant frequencies for the three controls were 1.8, 2.3, and 7.3 x 10(-6), and those for the two survivors (who had received radiation doses of 2.46 and 2.15 Gy, based upon the revised atomic bomb shielded kerma estimates) were 9.3 and 14.4 x 10(-6), respectively. Fourteen (13%) of 105 mutant T-cell colonies from the controls showed various structural changes in the hprt gene. The frequency of mutants with hprt gene structural changes in one atomic bomb survivor, who exhibited a mutant frequency of 9.3 x 10(-6), was 26% (16/61), which was significantly higher than that of the controls. However, the frequency of structural changes in the other survivor (14%, 8/59) was not higher than that of the controls. Two sets of mutants (in total, eight mutants) from the survivor, who showed a significantly higher frequency of mutants with hprt gross alterations than did the controls, had the same hprt changes and the same rearrangements of T-cell receptor (TcR) beta- and gamma-chain genes, indicating a clonal expansion from one progenitor mutant. This phenomenon may reflect an in vivo recovery process of T cells in the periphery after exposure to atomic bomb radiation. However, when comparing the frequency of mutations, these two sets of mutants should be reduced. After reducing the total number of mutants from the number of gross hprt changes, the frequency was not significantly higher than that of the controls.
- Research Organization:
- Radiation Effects Research Foundation, Hiroshima (Japan)
- OSTI ID:
- 6497005
- Journal Information:
- Environ. Mol. Mutagen.; (United States), Vol. 13:1
- Country of Publication:
- United States
- Language:
- English
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LYMPHOCYTES
GENETIC RADIATION EFFECTS
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RADIOINDUCTION
A-BOMB SURVIVORS
DNA REPAIR
GENES
HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE
IN VIVO
MUTATION FREQUENCY
RADIATION INDUCED MUTANTS
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
ENZYMES
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GLYCOSYL TRANSFERASES
HUMAN POPULATIONS
LEUKOCYTES
MATERIALS
MUTANTS
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RECOVERY
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SOMATIC CELLS
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560151* - Radiation Effects on Animals- Man