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Molecular analyses of in vivo hprt mutations in human T-lymphocytes: IV. Studies in newborns

Journal Article · · Environmental and Molecular Mutagenesis; (USA)
; ;  [1]
  1. Univ. of Vermont, Burlington (USA)
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In order to characterize in vivo gene mutations that occur during fetal development, molecular analyses were undertaken of mutant 6-thioguanine resistant T-lymphocytes isolated from placental cord blood samples of 13 normal male newborns. These mutant T-cells were studied to define hypoxanthine-guanine phosphoribosyltransferase (hprt) gene structural alterations and to determine T-cell receptor (TCR) gene rearrangement patterns. Structural hprt alterations, as shown by Southern blot analyses, occurred in 85% of these mutant clones. These alterations consisted mostly of deletion of exons 2 and 3. These findings contrast with the 10-20% of gross structural alterations occurring randomly across the entire gene previously reported for T-cell mutants isolated from normal young adults. Iterative analyses of hprt structural alterations and TCR gene rearrangement patterns show that approximately one-third of the newborn derived mutants may have originated as pre- or intrathymic hprt mutations. This too contrasts with previous findings in adults where the background in vivo hprt mutations appeared to originate in postthymic T-lymphocytes.

OSTI ID:
6896780
Journal Information:
Environmental and Molecular Mutagenesis; (USA), Journal Name: Environmental and Molecular Mutagenesis; (USA) Vol. 14:4; ISSN 0893-6692; ISSN EMMUE
Country of Publication:
United States
Language:
English

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Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
AGE GROUPS
AMINES
ANIMAL CELLS
ANIMALS
AROMATICS
AZAARENES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CHILDREN
CONNECTIVE TISSUE CELLS
ENZYMES
GENE MUTATIONS
GLYCOSYL TRANSFERASES
GUANINE
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE
INFANTS
LEUKOCYTES
LYMPHATIC SYSTEM
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
MEMBRANE PROTEINS
MUTATIONS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PENTOSYL TRANSFERASES
PRIMATES
PROTEINS
PURINES
RECEPTORS
SOMATIC CELLS
THIOLS
THYMUS
TRANSFERASES
VERTEBRATES