Mechanisms of mutagenesis: analysis through the use of alcohol dehydrogenase in Drosophila. Progress report, February 1982-January 1983

Technical Report · Sat Jan 01 04:00:00 EST 1983

OSTI ID:6489713

Sofer, W H

It has been shown that: (1) one EMS-induced, ADH-negative mutant called CyO/sup nB/, has a single base change - an AT to a GC bp - that results in the production of a premature termination codon in th ADH gene; (2) all formaldehyde-generated Adh mutants detected to date are deletions, most large enough to affect genes outside of the Adh locus as well as Adh; (3) four formaldehyde-generated mutants, though originally to be point mutants, were found, by DNA sequencing, to be small deletions ranging in size from 6 to 34 base pairs (bp) in length; and (4) three of these small deletions were found to occur between two bp direct repeats, and two bore, in addition to the deletions, additional changes adjoining the deleted DNA.

• Research Organization: Rutgers--the State Univ., Piscataway, NJ (USA). Waksman Inst. of Microbiology
• DOE Contract Number: AC02-81EV10566
• OSTI ID: 6489713
• Report Number(s): DOE/EV/10566-5; ON: DE83006976
• Country of Publication: United States
• Language: English

Related Subjects

550400 -- Genetics
560304* -- Chemicals Metabolism & Toxicology-- Invertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALDEHYDES
ANIMALS
ARTHROPODS
DIPTERA
DNA
DROSOPHILA
EMS
ENZYMES
ESTERS
FLIES
FORMALDEHYDE
FRUIT FLIES
GENE MUTATIONS
INSECTS
INVERTEBRATES
MOLECULAR STRUCTURE
MUTAGENESIS
MUTAGENS
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXIDOREDUCTASES
SULFONIC ACID ESTERS