Mechanisms of mutagenesis-analysis through the use of alcohol dehydrogenase in drosophila. Progress report

Technical Report · Sat Jan 01 04:00:00 EST 1983

OSTI ID:5856251

Sofer, W H

Several chemically induced mutants in the ADH gene have been characterized at the molecular level. We have been able to show that: one EMS induced, ADH negative mutant called CyO/sup nB/, has undergone a single base change - an AT to a GC bp - that results in the appearance of a premature termination codon within the ADH gene; the ADH genes from two other EMS induced mutants have been cloned; all formaldehyde-generated Adh mutants detected to date have turned out to be deletions; four formaldehyde-generated mutants, thought originally to be point mutants, were found, by DNA sequencies, to be small deletions ranging in size from 6 to 34 base pairs (bp) in length; and three of these small deletions were found to occur between two bp direct repeats. One deletion occurred at the base of a quasipalindromic sequence. Two deletions showed additional base changes in sequences adjoining the deleted DNA.

• Research Organization: Rutgers--the State Univ., Piscataway, NJ (USA). Waksman Inst. of Microbiology
• DOE Contract Number: AC02-81EV10566
• OSTI ID: 5856251
• Report Number(s): DOE/EV/10566-6; ON: DE83016273
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ARTHROPODS
CLONING
DIPTERA
DNA
DROSOPHILA
FLIES
FRUIT FLIES
GENES
INSECTS
INVERTEBRATES
MOLECULAR STRUCTURE
MUTANTS
NUCLEIC ACIDS
ORGANIC COMPOUNDS