Isolation and fluorescence in situ hybridization mapping of 60 cosmid clones on human chromosome 18
- National Institute of Genetics, Yata (Japan)
The authors have mapped 60 new cosmids on the short and long arms of chromosome 18 either by R- or by DAPI-banding and simultaneous fluorescence in situ hybridization. These markers were isolated from hybrid MS126-21 made from a human-rodent hybrid cell line that retained human chromosome 18. Twenty-two of the cosmid probes map on the short arm, and 31 probes cluster in the distal half of the long arm between bands 18q21.1 and 18q23, while 7 other probes are mapped more proximal to the centromere around bands 18q1.1-q12.3. The technique of fluorescence in situ hybridization has proven to be a very efficient methodology for gene mapping. These 60 probes will be useful in the elucidation of genetic alterations associated with diseases such as tetrasomy 18p syndrome, 18q- syndromes, and colorectal cancer. 19 refs., 2 figs.
- OSTI ID:
- 6483166
- Journal Information:
- Genomics; (United States), Journal Name: Genomics; (United States) Vol. 19:3; ISSN GNMCEP; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
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