Fluorescence in situ hybridization mapping of human chromosome 19: Cytogenetic band location of 540 cosmids and 70 genes or DNA markers

Journal Article · Fri Jan 01 04:00:00 EST 1993 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1993.1021· OSTI ID:6717225

Trask, B; Fertitta, A; Christensen, M; Bergmann, A; Copeland, A; Jong, P de; Mohrenweiser, H; Olsen, A; Carrano, A; Tynan, K ^[1]; Youngblom, J ^[2]

---

1. Lawrence Livermore National Lab., CA (United States)
2. California State Univ., Turlock (United States)

We report here the band location of 540 cosmids mapped to chromosome 19. The cosmids were mapped by fluorescence in situ hybridization (FISH) relative to chromosomal bands produced by DAPI/actinomycin staining. The cosmids are distributed throughout the chromosome, with a sampling bias for the q-arm. A detailed analysis of the distribution of three different subtelomeric and 22 pericentromeric chromosome 19 cosmids on other chromosomes is also reported. Colony hybridization identified 142 cosmids that contain sequences representing genes or DNA markers that map to chromosome 19. FISH mapping of these cosmids sublocalizes a total of 70 genes and DNA markers on chromosome 19, revises the previously published map assignments of 2 genes, and narrows the location of over 20 markers. 83 refs., 5 figs., 3 tabs.

• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 6717225
• Journal Information: Genomics; (United States), Journal Name: Genomics; (United States) Vol. 15:1; ISSN GNMCEP; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
CHROMOSOMES
COSMIDS
DISTRIBUTION
DNA
FLUORESCENCE
GENES
GENETIC MAPPING
HUMAN CHROMOSOME 19
HUMAN CHROMOSOMES
HYBRIDIZATION
LUMINESCENCE
MAPPING
NUCLEIC ACIDS
ORGANIC COMPOUNDS