Copper binding affinity of rainbow trout (Oncorhynchus mykiss) and brook trout (Salvelinus fontinalis) gills: Implications for assessing bioavailable metal

Journal Article · Tue Jun 01 04:00:00 EDT 1999 · Environmental Toxicology and Chemistry

DOI:https://doi.org/10.1897/1551-5028(1999)018<1180:CBAORT>2.3.CO;2· OSTI ID:6479805

MacRae, R K; Smith, D E; Swoboda-Colberg, N; Meyer, J S; Bergman, H L ^[1]

---

1. Univ. of Wyoming, Laramie, WY (United States). Dept. of Zoology and Physiology

In this study, the authors determined the conditional stability constant (log K[prime]) of copper for the gills of rainbow trout (Oncorhynchus mykiss; RBT) and brook trout (Salvelinus fontinalis; BT). Using toxicity-based complexation bioassays, which measure the effect of competing organic ligands on copper toxicity, the RBT gill copper log K[prime] range was 6.4 to 7.2. Using a Scatchard analysis of gill Cu accumulation, the RBT log K[prime] was 7.50 and the BT log K[prime] was 7.25. The close agreement in RBT log K[prime] values between these two methods suggests that measurement of gill copper accumulation is an acceptable alternative for determining a toxicity-based gill copper binding affinity. The results also suggest that there is either a single gill copper binding component or, more realistically, multiple components with similar binding properties that function collectively to define a single toxicologically relevant copper conditional stability constant. These results suggest analytical approaches to measuring bioavailable metal concentrations, such as geochemical modeling where biological ligands are included in speciation calculations, may adequately simulate complex biological ligands. A method to convert gill copper accumulation to a bioavailable water criterion is also discussed.

• OSTI ID: 6479805
• Journal Information: Environmental Toxicology and Chemistry, Journal Name: Environmental Toxicology and Chemistry Vol. 18:6; ISSN 0730-7268; ISSN ETOCDK
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
540320* -- Environment
Aquatic-- Chemicals Monitoring &amp; Transport-- (1990-)
560300 -- Chemicals Metabolism &amp; Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AQUATIC ORGANISMS
BIOLOGICAL ACCUMULATION
BIOLOGICAL AVAILABILITY
BIOLOGICAL INDICATORS
COPPER
ELEMENTS
FISHES
GILLS
METALS
POLLUTION
RESPIRATORY SYSTEM
TOXICITY
TRANSITION ELEMENTS
TROUT
VERTEBRATES
WATER POLLUTION