Evolution of primary and secondary structures in 5S and 5. 8S rRNA. [Bombyx mori]
Thesis/Dissertation
·
OSTI ID:6478815
The secondary structure of Bombyx mori 5S rRNA was studied using the sing-strand specific S1 nuclease and the base pair specific cobra venom ribonuclease. The RNA was end-labeled with (/sup 32/P) at either the 5' or 3' end and sequenced using enzymatic digestion techniques. These enzymatic data coupled with thermodynamic structure prediction were used to generate a secondary structure for 5S rRNA. A computer algorithm has been implemented to aid in the comparison of a large set of homologous RNAs. Eukaryotic 5S rRNA sequences from thirty four diverse species were compared by (1) alignment or the sequences, (2) the positions of substitutions were located with respect to the aligned sequence and secondary structure, and (3) the R-Y model of base stacking was used to study stacking pattern relationships in the structure. Eukaryotic 5S rRNA was found to have significant sequence variation throughout much of the molecule while maintaining a relatively constant secondary structure. A detailed analysis of the sequence and structure variability in each region of the molecule is presented.
- Research Organization:
- Syracuse Univ., NY (USA)
- OSTI ID:
- 6478815
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALGORITHMS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
COMPUTER CALCULATIONS
DAYS LIVING RADIOISOTOPES
ENZYMES
ESTERASES
GENETIC MAPPING
HYDROLASES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAPPING
MATHEMATICAL LOGIC
MATHEMATICAL MODELS
MOLECULAR STRUCTURE
NUCLEASES
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
RADIOISOTOPES
RIBOSOMAL RNA
RNA
TRACER TECHNIQUES
59 BASIC BIOLOGICAL SCIENCES
ALGORITHMS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
COMPUTER CALCULATIONS
DAYS LIVING RADIOISOTOPES
ENZYMES
ESTERASES
GENETIC MAPPING
HYDROLASES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAPPING
MATHEMATICAL LOGIC
MATHEMATICAL MODELS
MOLECULAR STRUCTURE
NUCLEASES
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
RADIOISOTOPES
RIBOSOMAL RNA
RNA
TRACER TECHNIQUES