Determination of steady state and nonsteady-state glycerol kinetics in humans using deuterium-labeled tracer

Journal Article · Wed Apr 01 00:00:00 EST 1987 · J. Lipid Res.; (United States)

OSTI ID:6478419

Beylot, M; Martin, C; Beaufrere, B; Riou, J P; Mornex, R

Using deuterium-labeled glycerol as tracer and gas-liquid chromatography-mass spectrometry techniques for the determination of isotopic enrichment, we have developed a simple and ethically acceptable method of determining glycerol appearance rate in humans under steady-state and nonsteady-state conditions. In normal subjects, the appearance rate of glycerol in the post-absorptive state was 2.22 +/- 0.20 mumol X kg-1 X min-1, a value in agreement with those reported in studies with radioactively labeled tracers. The ratio nonesterified fatty acid (NEFA) appearance rate/glycerol appearance rate ranged from 1.95 to 3.40. In insulin-dependent diabetic patients with a mild degree of metabolic control, the appearance rate of glycerol was 2.48 +/- 0.29 mumol X kg-1 X min-1. The volume of distribution of glycerol, determined by the bolus injection technique, was (mean) 0.306 l X kg-1 in normal subjects and 0.308 l X kg-1 in insulin-independent diabetic patients. To evaluate the usefulness of the method for determination of glycerol kinetics in nonsteady-state conditions, we infused six normal subjects with natural glycerol and calculated the isotopically determined glycerol appearance rate using a single compartment model (volume of distribution 0.31 l X kg-1). During these tests, the expected glycerol appearance rates were successively 5.03 +/- 0.33, 7.48 +/- 0.39, 9.94 +/- 0.34, 7.48 +/- 0.39, and 5.03 +/- 0.33 mumol +/- kg-1 X min-1, whereas the corresponding isotopically determined appearance rates were 4.62 +/- 0.45, 6.95 +/- 0.56, 10.85 +/- 0.51, 7.35 +/- 0.34, and 5.28 +/- 0.12 mumol X kg-1 X min-1.

• Research Organization: Faculte de Medecine Alexis Carrel, Lyon, France
• OSTI ID: 6478419
• Journal Information: J. Lipid Res.; (United States), Vol. 4
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLYCEROL
BIOCHEMICAL REACTION KINETICS
DEUTERIUM
DIABETES MELLITUS
GAS CHROMATOGRAPHY
INSULIN
MAN
STEADY-STATE CONDITIONS
TRACER TECHNIQUES
ALCOHOLS
ANIMALS
CHROMATOGRAPHY
DISEASES
ENDOCRINE DISEASES
HORMONES
HYDROGEN ISOTOPES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
MAMMALS
METABOLIC DISEASES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PRIMATES
REACTION KINETICS
SEPARATION PROCESSES
STABLE ISOTOPES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques