Photosynthetic metabolism of malate and aspartate in Flaveria trinervia a C/sub 4/ dicot

Thesis/Dissertation · Wed Jan 01 00:00:00 EST 1986

OSTI ID:6477975

Moore, B A

C/sub 4/ species are known to vary in their apparent relative use of malate and aspartate to mediate carbon flux through the C/sub 4/ cycle. These studies investigate some of the adjustments in photosynthetic carbon metabolism that occur during a dark to light transition and during expansion of leaves of Flaveria trinervia, a C/sub 4/ dicot. Enzyme localization studies with isolated leaf mesophyll and bundle sheath protoplasts, indicated that both C/sub 4/ acids are formed in the mesophyll chloroplast, and that aspartate is metabolized to malate in the bundle sheath chloroplast prior to decaroxylation there. During photosynthetic induction, the partitioning of /sup 14/CO/sub 2/ between malate and aspartate showed a single oscillation of increased aspartate labelling after 5 min of illumination. Turnover of (4-14C) (malate plus aspartate) was slow initially during illumination, prior to establishment of active pools of C/sub 4/ cycle metabolites.

• Research Organization: Washington State Univ., Pullman (USA)
• OSTI ID: 6477975
• Resource Relation: Other Information: Thesis (Ph. D)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ASPARTIC ACID
METABOLISM
C4 SPECIES
PHOTOSYNTHESIS
MALIC ACID
PHOTOSYNTHETIC REACTION CENTERS
BIOCHEMICAL REACTION KINETICS
CARBON 14 COMPOUNDS
TRACER TECHNIQUES
AMINO ACIDS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
HYDROXY ACIDS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHOTOCHEMICAL REACTIONS
REACTION KINETICS
SYNTHESIS
550501* - Metabolism- Tracer Techniques