Capacity for C/sub 4/ photosynthesis among relatively advanced species of Flaveria. [Flaveria vaginata; Flaveria palmeri; Flaveria australasica; Flaveria bidentis; Flaveria trinervia]
Leaves of 5 summer-grown Flaveria species, all thought to be C/sub 4/ plants, were exposed to /sup 14/CO/sub 2/ for 3-16 s. Analysis of /sup 14/C-products and extrapolation of labelling curves to zero time allowed estimation of the percentage of CO/sub 2/ incorporated via the C/sub 4/ cycle. This value was about 90% in F. vaginata and F. palmeri, 95% in F. australasica, 97% in F. bidentis, and 100% in F. trinervia. Localization of leaf carboxylases and decarboxylase was examined after purifying mesophyll and bundle sheath protoplasts by a simplified procedure. All species except F. trinervia showed some enrichment of RuBisCO to NADP-malic enzyme in mesophyll preparations. Mesophyll RuBisCO activities (up to 50 ..mu..mol/mg Chl x h) constituted less than or equal to7% of the total leaf RuBisCO (corrected for Chl distribution). Incomplete compartmentation of NADP-malic enzyme of direct entry of CO/sub 2/ into the bundle sheath may also occur in F. vaginata and F. palmeri. The data indicate that at least these 2 species (along with F. brownii from previous results) should be classified as less advanced, C/sub 4/-like species.
- Research Organization:
- Washington State Univ., Pullman
- OSTI ID:
- 6073212
- Report Number(s):
- CONF-8707108-; TRN: 87-037213
- Journal Information:
- Plant Physiol., Suppl.; (United States), Vol. 83:4; Conference: Annual meeting of the American Society of Plant Physiologists, St. Louis, MO, USA, 19 Jul 1987
- Country of Publication:
- United States
- Language:
- English
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Photosynthetic metabolism of malate and aspartate in Flaveria trinervia a C/sub 4/ dicot
Environmental effects on photorespiration of C sub 3 -C sub 4 species. I. Influence of CO sub 2 and O sub 2 during growth on photorespiratory characteristics and leaf anatomy. [Flaveria pringlei; Flaveria flroidana; Flaveria trinervia; Panicum milloides; Panicum laxum]
Related Subjects
C4 SPECIES
ENZYME ACTIVITY
PHOTOSYNTHESIS
ENZYMES
SUBCELLULAR DISTRIBUTION
BIOCHEMICAL REACTION KINETICS
CARBON 14 COMPOUNDS
CARBON DIOXIDE
CARBOXYLASE
CHLOROPHYLL
DECARBOXYLASES
PLANT CELLS
RIBULOSE DIPHOSPHATE CARBOXYLASE
TRACER TECHNIQUES
CARBON COMPOUNDS
CARBON OXIDES
CARBON-CARBON LYASES
CARBOXY-LYASES
CARBOXYLIC ACIDS
CHALCOGENIDES
CHEMICAL REACTIONS
DISTRIBUTION
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LYASES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PHOTOCHEMICAL REACTIONS
PHYTOCHROMES
PIGMENTS
PORPHYRINS
PROTEINS
REACTION KINETICS
SYNTHESIS
550201* - Biochemistry- Tracer Techniques