Lactoferrin derived from neutrophils inhibits the mixed lymphocyte reaction
Journal Article
·
· Blood; (United States)
OSTI ID:6472411
The effects of the products of phagocytosing neutrophils on lymphocyte transcriptional activity have been investigated using allogeneic mixed lymphocyte cultures (MLC). When MLCs are grown crowded in round-bottomed wells, uptake of tritiated uridine is inhibited by the presence of neutrophil products. Similarly, inhibitory activity is shown by purified lactoferrin. The inhibitory factor released by neutrophils is positively identified as lactoferrin, since its activity is lost in the presence of specific antibody. In addition, inhibitory activity is lost when lactoferrin is removed by a monoclonal antibody (MoAb) column and is recovered with lactoferrin from the column. The active form of lactoferrin carries iron and the apoprotein is inactive. MLCs crowded in round-bottomed wells take up considerably more uridine than when spread in flat-bottomed wells, and it is this enhanced transcriptional activity that is inhibited by neutrophil-derived lactoferrin. Enhanced uridine uptake by crowded cultures is due to factors that can be transferred in the supernatants to promote uridine uptake by spread cultures. Neutrophil lactoferrin inhibits both production and effect of the transferable factor(s).
- Research Organization:
- City Hospital, Nottingham, England
- OSTI ID:
- 6472411
- Journal Information:
- Blood; (United States), Journal Name: Blood; (United States) Vol. 5; ISSN BLOOA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CONNECTIVE TISSUE CELLS
CULTURE MEDIA
ELEMENTS
GLOBULINS
GLUCOPROTEINS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INHIBITION
IRON
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LACTOFERRIN
LEUKOCYTES
LYMPHOCYTES
MATERIALS
METALLOPROTEINS
METALS
NEUTROPHILS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHAGOCYTOSIS
PROTEINS
PYRIMIDINES
RIBOSIDES
SACCHARIDES
SOMATIC CELLS
TRACER TECHNIQUES
TRANSCRIPTION
TRANSITION ELEMENTS
TRITIUM COMPOUNDS
URACILS
URIDINE
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CONNECTIVE TISSUE CELLS
CULTURE MEDIA
ELEMENTS
GLOBULINS
GLUCOPROTEINS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INHIBITION
IRON
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LACTOFERRIN
LEUKOCYTES
LYMPHOCYTES
MATERIALS
METALLOPROTEINS
METALS
NEUTROPHILS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHAGOCYTOSIS
PROTEINS
PYRIMIDINES
RIBOSIDES
SACCHARIDES
SOMATIC CELLS
TRACER TECHNIQUES
TRANSCRIPTION
TRANSITION ELEMENTS
TRITIUM COMPOUNDS
URACILS
URIDINE