Synergistic effect of aphidicolin on the yield of X-ray-induced chromosome aberrations throughout the cell cycle in JU56 cells

Journal Article · Mon Jun 01 04:00:00 EDT 1987 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3577006· OSTI ID:6471977

Moore, R C; Bender, M A

The effect of a 2-h post-treatment with aphidicolin at a dose sufficient to inhibit DNA synthesis on the yield of X-ray-induced chromosomal aberrations throughout the cell cycle was measured. Exposure to aphidicolin during and after irradiation brought about an increase in exchanges in cells irradiated in G2, in sister unions only in cells irradiated in S, and in all chromosome aberration types (fragments, sister unions, and dicentrics) in cells irradiated in G1. It is suggested that, during G1 and G2 but not during S inhibiting the repair enzyme alpha-polymerase brings about the conversion of some X-ray-induced DNA lesions to double-strand which can then take part in aberrations.

• Research Organization: Cancer Institute, Melbourne, Australia
• OSTI ID: 6471977
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 3; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANTIMITOTIC DRUGS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CYCLE
CHROMOSOMAL ABERRATIONS
DNA REPLICATION
DRUGS
ELECTROMAGNETIC RADIATION
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
IONIZING RADIATIONS
MUTATIONS
NUCLEIC ACID REPLICATION
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY EFFECTS
SYNERGISM
X RADIATION